CPLM-005845

Genbank Nucleotide ID

Neutrophil gelatinase-associated lipocalin

Protein Synonyms/Alias

NGAL; Alpha-2-microglobulin-related protein; Alpha-2U globulin-related protein; Lipocalin-2; Siderocalin LCN2; p25

Rattus norvegicus (Rat)

Position	Peptide	Type	References
150	FQKTSENKQYFKVTL	acetylation	[1]

[1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]

Functional Description

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth (By similarity).

BINDING 126 126 Catecholate-type ferric siderophore (By
BINDING 145 145 Catecholate-type ferric siderophore (By
BINDING 154 154 Catecholate-type ferric siderophore (By
MOD_RES 21 21 Pyrrolidone carboxylic acid (By
CARBOHYD 85 85 N-linked (GlcNAc...) (Potential).
DISULFID 96 195

3D-structure; Apoptosis; Complete proteome; Disulfide bond; Glycoprotein; Immunity; Innate immunity; Ion transport; Iron; Iron transport; Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; IDA:RGD.
GO:0005615; C:extracellular space; IDA:RGD.
GO:0005506; F:iron ion binding; ISS:UniProtKB.
GO:0042803; F:protein homodimerization activity; IDA:RGD.
GO:0036094; F:small molecule binding; IEA:InterPro.
GO:0005215; F:transporter activity; IEA:InterPro.
GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
GO:0070301; P:cellular response to hydrogen peroxide; IDA:RGD.
GO:0071347; P:cellular response to interleukin-1; IEP:RGD.
GO:0071222; P:cellular response to lipopolysaccharide; IEP:RGD.
GO:0031669; P:cellular response to nutrient levels; IEP:RGD.
GO:0071356; P:cellular response to tumor necrosis factor; IEP:RGD.
GO:0045087; P:innate immune response; ISS:UniProtKB.
GO:0006811; P:ion transport; IEA:UniProtKB-KW.
GO:0031346; P:positive regulation of cell projection organization; IDA:RGD.
GO:0010628; P:positive regulation of gene expression; IDA:RGD.
GO:0070207; P:protein homotrimerization; IDA:RGD.
GO:0042981; P:regulation of apoptotic process; ISS:UniProtKB.
GO:0042493; P:response to drug; IMP:RGD.
GO:0009635; P:response to herbicide; IDA:RGD.
GO:0009615; P:response to virus; IEA:Compara.
GO:0015891; P:siderophore transport; ISS:UniProtKB.

IPR012674; Calycin.
IPR011038; Calycin-like.
IPR002345; Lipocalin.
IPR000566; Lipocln_cytosolic_FA-bd_dom.
IPR003087; N_gelatinase.

PR00179; LIPOCALIN.
PR01275; NGELATINASE.