CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037899
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 mRNA export factor 
Protein Synonyms/Alias
  
Gene Name
 RAE1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
212RRIESPLKHQHRCVAubiquitination[1, 2]
222HRCVAIFKDKQNKPTubiquitination[1]
224CVAIFKDKQNKPTGFubiquitination[1, 3]
227IFKDKQNKPTGFALGubiquitination[2, 4]
252INPPNPAKDNFTFKCacetylation[2]
252INPPNPAKDNFTFKCubiquitination[1, 2, 3, 4, 5, 6]
258AKDNFTFKCHRSNGTubiquitination[1, 2]
302GRFSFWDKDARTKLKubiquitination[1, 3, 4]
309KDARTKLKTSEQLDQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 437 AA 
Protein Sequence
MSLFGTTSGF GTSGTSMFGS ATTDNHNPMK DIEVTSSPDD SIGCLSFSPP TLPGNFLIAG 60
SWANDVRCWE VQDSGQTIPK AQQMHTGPVL DVCWSDDGSK VFTASCDKTA KMWDLSSNQA 120
IQIAQHDAPV KTIHWIKAPN YSCVMTGSWD KTLKFWDTRS SNPMMVLQLP ERCYCADVIY 180
PMAVVATAER GLIVYQLENQ PSEFRRIESP LKHQHRCVAI FKDKQNKPTG FALGSIEGRV 240
AIHYINPPNP AKDNFTFKCH RSNGTNTSAP QDIYAVNGIA FHPVHGTLAT VGSDGRFSFW 300
DKDARTKLKT SEQLDQPISA CCFNHNGNIF AYASSYDWSK VRTPGPALGA PRQQSQALGS 360
EGLGLSCDSS QDSRVSLGRS PSPPCKGKLQ DFLKWLWGFS TAFGQNPAHR KCSASVSHYG 420
LLCSWAQKHR QAGGCTL 437 
Gene Ontology
  
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.