CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032142
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 PAS domain-containing serine/threonine-protein kinase 
Protein Synonyms/Alias
 cDNA FLJ61481, highly similar to PAS domain-containing serine/threonine-protein kinase (EC 2.7.11.1) 
Gene Name
 PASK 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
100FPLSLKLKSQPSSEEubiquitination[1, 2]
378WAGAAVAKPQAKGQLubiquitination[1, 2]
714LFCCWLVKDLLHSQRubiquitination[2]
906AVGYLRLKDIIHRDIubiquitination[2]
1054EEVFRVNKPESGVLSubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1107 AA 
Protein Sequence
MKRMRQERRL CCVVVLEPVE RVSTWVAFQS DGTVTSCDSL FAHLHGYVSG EDVAGQHITD 60
LIPSVQLPPS GQHIPKNLKI QRSVGRARDG TTFPLSLKLK SQPSSEEATT GEAAPVSGYR 120
ASVWVFCTIS GLITLLPDGT IHGINHSFAL TLFGYGKTEL LGKNITFLIP GFYSYMDLAY 180
NSSLQLPDLA SCLDVGNESG CGERTLDPWQ GQDPAEGGQD PRINVVLAGG HVVPRDEIRK 240
LMESQDIFTG TQTELIAGGQ LLSCLSPQPA PGVDNVPEGS LPVHGEQALP KDQQITALGR 300
EEPVAIESPG QDLLGESRSE PVDVKPFASC EDSEAPVPAE DGGSDAGMCG LCQKAQLERM 360
GVSGPSGSDL WAGAAVAKPQ AKGQLAGGSL LMHCPCYGSE WGLWWRSQDL APSPSGMAGL 420
SFGTPTLDEP WLGVENDREE LQTCLIKEQL SQLSLAGALD VPHAELVPTE CQAVTAPVSS 480
CDLGGRDLCG GCTGSSSACY ALATDLPGGL EAVEAQEVDV NSFSWNLKEL FFSDQTDQTS 540
SNCSCATSEL RETPSSLAVG SDPDVGSLQE QGSCVLDDRE LLLLTGTCVD LGQGRRFRES 600
CVGHDPTEPL EVCLVSSEHY AASDRESPGH VPSTLDAGPE DTCPSAEEPR LNVQVTSTPV 660
IVMRGAAGLQ REIQEGAYSG SCYHRDGLRL SIQFEVRRVE LQGPTPLFCC WLVKDLLHSQ 720
RDSAARTRLF LASLPGSTHS TAAELTGPSL VEVLRARPWF EEPPKAVELE GLAACEGEYS 780
QKYSTMSPLG SGAFGFVWTA VDKEKNKEVV VKFIKKEKVL EDCWIEDPKL GKVTLEIAIL 840
SRVEHANIIK VLDIFENQGF FQLVMEKHGS GLDLFAFIDR HPRLDEPLAS YIFRQLVSAV 900
GYLRLKDIIH RDIKDENIVI AEDFTIKLID FGSAAYLERG KLFYTFCGTI EYCAPEVLMG 960
NPYRGPELEM WSLGVTLYTL VFEENPFCEL EETVEAAIHP PYLVSKELMS LVSGLLQPVP 1020
ERRTTLEKLV TDPWVTQPVN LADYTWEEVF RVNKPESGVL SAASLEMGNR SLSDVAQAQE 1080
LCGGPVPGEA PNGQGCLHPG DPRLLTS 1107 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:InterPro.
 GO:0004871; F:signal transducer activity; IEA:InterPro. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000014; PAS.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00091; PAS
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS