CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004873
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATP-dependent RNA helicase DBP5 
Protein Synonyms/Alias
 DEAD box protein 5; Helicase CA5/6; Ribonucleic acid-trafficking protein 8 
Gene Name
 DBP5 
Gene Synonyms/Alias
 RAT8; YOR046C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
21SLKIDNEKEDTSEVSubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 ATP-dependent RNA helicase associated with the nuclear pore complex and essential for mRNA export from the nucleus. May participate in a terminal step of mRNA export through the removal of proteins that accompany mRNA through the nucleopore complex. Contributes to the blocking of bulk poly(A)+ mRNA export in ethanol-stressed cells. May also be involved in early transcription. 
Sequence Annotation
 DOMAIN 125 292 Helicase ATP-binding.
 DOMAIN 303 480 Helicase C-terminal.
 NP_BIND 138 145 ATP (By similarity).
 MOTIF 92 120 Q motif.
 MOTIF 239 242 DEAD box.
 MOD_RES 86 86 Phosphoserine.
 MOD_RES 93 93 Phosphoserine.
 MOD_RES 162 162 Phosphoserine.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase; Membrane; mRNA transport; Nuclear pore complex; Nucleotide-binding; Nucleus; Phosphoprotein; Protein transport; Reference proteome; RNA-binding; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 482 AA 
Protein Sequence
MSDTKRDPAD LLASLKIDNE KEDTSEVSTK ETVKSQPEKT ADSIKPAEKL VPKVEEKKTK 60
QEDSNLISSE YEVKVKLADI QADPNSPLYS AKSFDELGLA PELLKGIYAM KFQKPSKIQE 120
RALPLLLHNP PRNMIAQSQS GTGKTAAFSL TMLTRVNPED ASPQAICLAP SRELARQTLE 180
VVQEMGKFTK ITSQLIVPDS FEKNKQINAQ VIVGTPGTVL DLMRRKLMQL QKIKIFVLDE 240
ADNMLDQQGL GDQCIRVKRF LPKDTQLVLF SATFADAVRQ YAKKIVPNAN TLELQTNEVN 300
VDAIKQLYMD CKNEADKFDV LTELYGLMTI GSSIIFVATK KTANVLYGKL KSEGHEVSIL 360
HGDLQTQERD RLIDDFREGR SKVLITTNVL ARGIDIPTVS MVVNYDLPTL ANGQADPATY 420
IHRIGRTGRF GRKGVAISFV HDKNSFNILS AIQKYFGDIE MTRVPTDDWD EVEKIVKKVL 480
KD 482 
Gene Ontology
 GO:0005934; C:cellular bud tip; IDA:SGD.
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0044614; C:nuclear pore cytoplasmic filaments; IDA:SGD.
 GO:0005844; C:polysome; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0003724; F:RNA helicase activity; IDA:SGD.
 GO:0008186; F:RNA-dependent ATPase activity; IDA:SGD.
 GO:0006406; P:mRNA export from nucleus; IGI:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0006415; P:translational termination; IGI:SGD. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS