CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019170
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mdm2-binding protein 
Protein Synonyms/Alias
 hMTBP 
Gene Name
 MTBP 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
51ANVYHLLKRSISASIubiquitination[1]
236LRNVIDSKELWRGKIubiquitination[1]
242SKELWRGKIQIWERKubiquitination[1]
267CLKGVTLKNFSTSNLubiquitination[1]
281LNTDFLAKKIIPSKDubiquitination[1]
483LALEECLKRRKLAKQubiquitination[1]
500TVSVAELKSLLVLTRubiquitination[1]
570QNLETFEKTKQKMRTubiquitination[2]
572LETFEKTKQKMRTGSubiquitination[1]
591SEQLLGHKEGPRDSIubiquitination[2]
604SITLLDAKELLKYFTubiquitination[1, 2, 3]
608LDAKELLKYFTSDGLubiquitination[1, 2]
627LQPLPIQKGEKTFVLubiquitination[1, 2, 3, 4]
630LPIQKGEKTFVLTPEubiquitination[1, 3, 5, 6]
642TPELSPGKLQVLPFEubiquitination[1, 2, 3]
866QRLFEISKFYLKDLKubiquitination[1]
884GLFEEMKKTANNNAVubiquitination[1]
904VLEKTSKK*******ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Inhibits cell migration in vitro and suppresses the invasive behavior of tumor cells (By similarity). May play a role in MDM2-dependent p53/TP53 homeostasis in unstressed cells. Inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of p53/TP53 and its subsequent degradation. 
Sequence Annotation
 REGION 521 904 Interaction with MDM2 (By similarity).
 MOD_RES 639 639 Phosphoserine.
 MOD_RES 703 703 Phosphoserine.
 MOD_RES 707 707 Phosphoserine.  
Keyword
 Cell cycle; Complete proteome; Growth arrest; Phosphoprotein; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 904 AA 
Protein Sequence
MDRYLLLVIW GEGKFPSAAS REAEHGPEVS SGEGTENQPD FTAANVYHLL KRSISASINP 60
EDSTFPACSV GGIPGSKKWF FAVQAIYGFY QFCSSDWQEI HFDTEKDKIE DVLQTNIEEC 120
LGAVECFEEE DSNSRESLSL ADLYEEAAEN LHQLSDKLPA PGRAMVDIIL LLSDKDPPKL 180
KDYLPTVGAL KHLREWYSAK ITIAGNHCEI NCQKIAEYLS ANVVSLEDLR NVIDSKELWR 240
GKIQIWERKF GFEISFPEFC LKGVTLKNFS TSNLNTDFLA KKIIPSKDKN ILPKVFHYYG 300
PALEFVQMIK LSDLPSCYMS DIEFELGLTN STKQNSVLLL EQISSLCSKV GALFVLPCTI 360
SNILIPPPNQ LSSRKWKEYI AKKPKTISVP DVEVKGECSS YYLLLQGNGN RRCKATLIHS 420
ANQINGSFAL NLIHGKMKTK TEEAKLSFPF DLLSLPHFSG EQIVQREKQL ANVQVLALEE 480
CLKRRKLAKQ PETVSVAELK SLLVLTRKHF LDYFDAVIPK MILRKMDKIK TFNILNDFSP 540
VEPNSSSLME TNPLEWPERH VLQNLETFEK TKQKMRTGSL PHSSEQLLGH KEGPRDSITL 600
LDAKELLKYF TSDGLPIGDL QPLPIQKGEK TFVLTPELSP GKLQVLPFEK ASVCHYHGIE 660
YCLDDRKALE RDGGFSELQS RLIRYETQTT CTRESFPVPT VLSPLPSPVV SSDPGSVPDG 720
EVLQNELRTE VSRLKRRSKD LNCLYPRKRL VKSESSESLL SQTTGNSNHY HHHVTSRKPQ 780
TERSLPVTCP LVPIPSCETP KLATKTSSGQ KSMHESKTSR QIKESRSQKH TRILKEVVTE 840
TLKKHSITET HECFTACSQR LFEISKFYLK DLKTSRGLFE EMKKTANNNA VQVIDWVLEK 900
TSKK 904 
Gene Ontology
 GO:0007050; P:cell cycle arrest; IEA:UniProtKB-KW.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0007089; P:traversing start control point of mitotic cell cycle; IEA:Compara. 
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS