UniProt Accession

 MED27_MOUSE; Q9DB40; A2AJV2; Q78JI3; Q9D707 

Genbank Protein ID

 AK005251; AK009764; AK147181; AL772213; AL929437; AL929437; AL772213; BC016537 

Genbank Nucleotide ID

 BAB23906.2; BAB26488.2; BAE27743.1; CAM13399.1; CAM13399.1; CAM26819.1; CAM26819.1; AAH16537.1 

Protein Name

 Mediator of RNA polymerase II transcription subunit 27 

Protein Synonyms/Alias

 Cofactor required for Sp1 transcriptional activation subunit 8; CRSP complex subunit 8; Mediator complex subunit 27 

Gene Name

 Med27 

Gene Synonyms/Alias

 Crsp8 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
50	ETLEGREKAFIANFQ	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). 

Sequence Annotation

  

Keyword

 Activator; Alternative splicing; Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 311 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0016592; C:mediator complex; IDA:MGI.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0003713; F:transcription coactivator activity; ISS:UniProtKB.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0019827; P:stem cell maintenance; IMP:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR021627; Mediator_Med27. 

Pfam

 PF11571; Med27 

SMART

  

PROSITE

  

PRINTS