CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002653
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Fumarate hydratase, mitochondrial 
Protein Synonyms/Alias
 Fumarase 
Gene Name
 FUM1 
Gene Synonyms/Alias
 YPL262W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
44EIHVPADKYWGAQTQacetylation[1]
58QRSFQNFKIGGARERacetylation[1]
94SLGGLDPKISKAIQQacetylation[1]
208KEFDHIVKIGRTHLQacetylation[1]
277KIAEQISKETGLKFQacetylation[1]
448IGYDAASKVAKNAHKubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
  
Sequence Annotation
 REGION 164 166 Substrate binding (By similarity).
 BINDING 126 126 Substrate (By similarity).
 MOD_RES 428 428 Phosphothreonine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Lyase; Mitochondrion; Phosphoprotein; Reference proteome; Transit peptide; Tricarboxylic acid cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 488 AA 
Protein Sequence
MLRFTNCSCK TFVKSSYKLN IRRMNSSFRT ETDAFGEIHV PADKYWGAQT QRSFQNFKIG 60
GARERMPLPL VHAFGVLKKS AAIVNESLGG LDPKISKAIQ QAADEVASGK LDDHFPLVVF 120
QTGSGTQSNM NANEVISNRA IEILGGKIGS KQVHPNNHCN QSQSSNDTFP TVMHIAASLQ 180
IQNELIPELT NLKNALEAKS KEFDHIVKIG RTHLQDATPL TLGQEFSGYV QQVENGIQRV 240
AHSLKTLSFL AQGGTAVGTG LNTKPGFDVK IAEQISKETG LKFQTAPNKF EALAAHDAIV 300
ECSGALNTLA CSLFKIAQDI RYLGSGPRCG YHELMLPENE PGSSIMPGKV NPTQNEALTQ 360
VCVQVMGNNA AITFAGSQGQ FELNVFKPVM IANLLNSIRL ITDAAYSFRV HCVEGIKANE 420
PRIHELLTKS LMLVTALNPK IGYDAASKVA KNAHKKGITL KESALELGVL TEKEFDEWVV 480
PEHMLGPK 488 
Gene Ontology
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005759; C:mitochondrial matrix; IDA:SGD.
 GO:0045239; C:tricarboxylic acid cycle enzyme complex; IEA:InterPro.
 GO:0004333; F:fumarate hydratase activity; IDA:SGD.
 GO:0006106; P:fumarate metabolic process; IMP:SGD.
 GO:0006099; P:tricarboxylic acid cycle; IMP:SGD. 
Interpro
 IPR005677; Fum_hydII.
 IPR024083; Fumarase/histidase_N.
 IPR018951; Fumarase_C_C.
 IPR000362; Fumarate_lyase.
 IPR020557; Fumarate_lyase_CS.
 IPR022761; Fumarate_lyase_N.
 IPR008948; L-Aspartase-like. 
Pfam
 PF10415; FumaraseC_C
 PF00206; Lyase_1 
SMART
  
PROSITE
 PS00163; FUMARATE_LYASES 
PRINTS
 PR00149; FUMRATELYASE.