CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001844
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mannose-6-phosphate isomerase 
Protein Synonyms/Alias
 Phosphohexomutase; Phosphomannose isomerase; PMI 
Gene Name
 manA 
Gene Synonyms/Alias
 pmi; b1613; JW1605 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
66RDVIESDKSTLLGEAacetylation[1]
76LLGEAVAKRFGELPFacetylation[1]
111NSEIGFAKENAAGIPacetylation[1]
195LNMQGEEKSRALAILacetylation[1]
203SRALAILKSALDSQQacetylation[1]
280LRAGLTPKYIDIPELacetylation[1, 2]
292PELVANVKFEAKPANacetylation[1]
362GSQQLQLKPGESAFIacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 
Functional Description
 Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide. 
Sequence Annotation
 ACT_SITE 274 274 By similarity.
 METAL 97 97 Zinc (By similarity).
 METAL 99 99 Zinc (By similarity).
 METAL 134 134 Zinc (By similarity).
 METAL 255 255 Zinc (By similarity).
 MOD_RES 280 280 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Isomerase; Metal-binding; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 391 AA 
Protein Sequence
MQKLINSVQN YAWGSKTALT ELYGMENPSS QPMAELWMGA HPKSSSRVQN AAGDIVSLRD 60
VIESDKSTLL GEAVAKRFGE LPFLFKVLCA AQPLSIQVHP NKHNSEIGFA KENAAGIPMD 120
AAERNYKDPN HKPELVFALT PFLAMNAFRE FSEIVSLLQP VAGAHPAIAH FLQQPDAERL 180
SELFASLLNM QGEEKSRALA ILKSALDSQQ GEPWQTIRLI SEFYPEDSGL FSPLLLNVVK 240
LNPGEAMFLF AETPHAYLQG VALEVMANSD NVLRAGLTPK YIDIPELVAN VKFEAKPANQ 300
LLTQPVKQGA ELDFPIPVDD FAFSLHDLSD KETTISQQSA AILFCVEGDA TLWKGSQQLQ 360
LKPGESAFIA ANESPVTVKG HGRLARVYNK L 391 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004476; F:mannose-6-phosphate isomerase activity; IDA:EcoCyc.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0009242; P:colanic acid biosynthetic process; IMP:EcoCyc.
 GO:0009298; P:GDP-mannose biosynthetic process; IMP:EcoCyc.
 GO:0019309; P:mannose catabolic process; IMP:EcoCyc. 
Interpro
 IPR001250; Man6P_Isoase-1.
 IPR016305; Mannose-6-P_Isomerase.
 IPR018050; Pmannose_isomerase-type1_CS.
 IPR014710; RmlC-like_jellyroll.
 IPR011051; RmlC_Cupin. 
Pfam
 PF01238; PMI_typeI 
SMART
  
PROSITE
 PS00965; PMI_I_1
 PS00966; PMI_I_2 
PRINTS
 PR00714; MAN6PISMRASE.