CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020963
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein phosphatase 1 regulatory subunit 12A 
Protein Synonyms/Alias
 Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1 
Gene Name
 Ppp1r12a 
Gene Synonyms/Alias
 Mypt1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
320PQKAFKNKETLIIEPacetylation[1]
320PQKAFKNKETLIIEPubiquitination[2]
815AYSRGLAKENEREGEubiquitination[2]
925PYSSRLEKDDSTDFKacetylation[1]
1014KADNQRLKDENGALIubiquitination[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity (By similarity). 
Sequence Annotation
 REPEAT 39 68 ANK 1.
 REPEAT 72 101 ANK 2.
 REPEAT 105 134 ANK 3.
 REPEAT 138 164 ANK 4.
 REPEAT 198 227 ANK 5.
 REPEAT 231 260 ANK 6.
 REGION 680 863 Interaction with ROCK2 (By similarity).
 MOTIF 35 38 KVKF motif.
 MOD_RES 67 67 (3S)-3-hydroxyasparagine; by HIF1AN (By
 MOD_RES 100 100 (3S)-3-hydroxyasparagine; by HIF1AN (By
 MOD_RES 226 226 (3S)-3-hydroxyasparagine; by HIF1AN (By
 MOD_RES 299 299 Phosphoserine (By similarity).
 MOD_RES 422 422 Phosphoserine (By similarity).
 MOD_RES 432 432 Phosphoserine (By similarity).
 MOD_RES 443 443 Phosphothreonine.
 MOD_RES 445 445 Phosphoserine; by NUAK1 (Probable).
 MOD_RES 472 472 Phosphoserine; by NUAK1 (By similarity).
 MOD_RES 473 473 Phosphoserine; by CDK1 (By similarity).
 MOD_RES 477 477 Phosphoserine (By similarity).
 MOD_RES 507 507 Phosphoserine.
 MOD_RES 601 601 Phosphoserine (By similarity).
 MOD_RES 666 666 Phosphoserine.
 MOD_RES 690 690 Phosphoserine; by PKA and PKG; in vitro
 MOD_RES 693 693 Phosphoserine; by PKA and PKG; in vitro
 MOD_RES 694 694 Phosphothreonine; by ROCK1; ROCK2;
 MOD_RES 851 851 Phosphoserine; by ROCK2 (By similarity).
 MOD_RES 861 861 Phosphoserine (By similarity).
 MOD_RES 870 870 Phosphoserine.
 MOD_RES 909 909 Phosphoserine; by NUAK1 (Probable).
 MOD_RES 994 994 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; ANK repeat; Complete proteome; Cytoplasm; Hydroxylation; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1029 AA 
Protein Sequence
MKMADAKQKR NEQLKRWIGS ETDLEPPVVK RQKTKVKFDD GAVFLAACSS GDTDEVLKLL 60
HRGADINYAN VDGLTALHQA CIDDNVDMVK FLVENGANIN QPDNEGWIPL HAAASCGYLD 120
IAEFLIGQGA HVGAVNSEGD TPLDIAEEEA MEELLQNEVN RQGVDIEAAR KEEERVMLRD 180
ARQWLNSGHI SDVRHAKSGG TALHVAAAKG YTEVLKLLIQ AGYDVNIKDY DGWTPLHAAA 240
HWGKEEACRI LVDNLCDMET VNKVGQTAFD VADEDILGYL EELQKKQTLL HSEKRDKKSP 300
LIESTANMEN NQPQKAFKNK ETLIIEPEKN ASRIESLEHE KADEEEEGKK DESSCSSEED 360
EEDDSESEAE TDKTKPMASV SNAHTSSTQA APAAVTAPTL SSNQGTPTSP VKKFPISTTK 420
ISPKEEERKD ESPASWRLGL RKTGSYGALA EISASKEAQK EKDTAGVMRS ASSPRLSSSL 480
DNKEKEKDNK GTRLAYVTPT IPRRLASTSD IEEKENRESS SLRTSSSYTR RKWEDDLKKN 540
SSINEGSTYH RSCSFGRRQD DLISCSVPST TSTPTVTSAA GLQRSLPSST STAAKTPPGS 600
SSAGTQSSTS NRLWAEDSTE KEKDSAPTAV TIPVAPTVVN AAAPSTTTLT TTTAGTVSEV 660
RERRRSYLTP VRDEESESQR KARSRQARQS RRSTQGVTLT DLQEAEKTIG RSRSTRTREQ 720
ENEEKEKEEK EKQDKEKQEE KKESEASRED EYKQKYSRTY DETYTRYRPV STSSSSAPSS 780
SSLSTLGSTL YASSQLNRPN SLVGITSAYS RGLAKENERE GEKKEEEKEG EDKSQPKSIR 840
ERRRPREKRR STGVSFWTQD SDENEQERQS DTEDGSSKRE TQTDSVSRYD SSSTSSSDRY 900
DSLLGRSASY SYLEDRKPYS SRLEKDDSTD FKKLYEQILA ENEKLKAQLH DTNMELTDLK 960
LQLEKATQRQ ERFADRSQLE MEKRERRALE RRISEMEEEL KMLPDLKADN QRLKDENGAL 1020
IRVISKLSK 1029 
Gene Ontology
 GO:0005813; C:centrosome; ISS:UniProtKB.
 GO:0043292; C:contractile fiber; IEA:Compara.
 GO:0000776; C:kinetochore; ISS:UniProtKB.
 GO:0072357; C:PTW/PP1 phosphatase complex; ISS:UniProtKB.
 GO:0071889; F:14-3-3 protein binding; ISS:UniProtKB.
 GO:0004857; F:enzyme inhibitor activity; ISS:UniProtKB.
 GO:0019208; F:phosphatase regulator activity; ISS:UniProtKB.
 GO:0051297; P:centrosome organization; ISS:UniProtKB.
 GO:0007067; P:mitosis; ISS:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0006470; P:protein dephosphorylation; IMP:MGI.
 GO:0030155; P:regulation of cell adhesion; ISS:UniProtKB.
 GO:0035507; P:regulation of myosin-light-chain-phosphatase activity; ISS:UniProtKB.
 GO:0046822; P:regulation of nucleocytoplasmic transport; IMP:MGI. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR017401; Pase-1_reg_su_12A/B/C_euk. 
Pfam
 PF12796; Ank_2 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 
PRINTS
 PR01415; ANKYRIN.