CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010453
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleobindin-2 
Protein Synonyms/Alias
 DNA-binding protein NEFA; Gastric cancer antigen Zg4; Prepronesfatin; Nesfatin-1 
Gene Name
 NUCB2 
Gene Synonyms/Alias
 NEFA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
90IKSGRLSKELDLVSHubiquitination[1, 2]
247DPNDFDPKTFFKLHDubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Calcium-binding protein. May have a role in calcium homeostasis. 
Sequence Annotation
 DOMAIN 241 276 EF-hand 1.
 DOMAIN 293 328 EF-hand 2.
 DNA_BIND 171 223 By similarity.
 REGION 213 420 Binds to necdin (By similarity).
 MOD_RES 257 257 Phosphoserine.
 MOD_RES 332 332 Phosphoserine.  
Keyword
 Alternative splicing; Calcium; Cleavage on pair of basic residues; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Endoplasmic reticulum; Golgi apparatus; Membrane; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Secreted; Signal. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 420 AA 
Protein Sequence
MRWRTILLQY CFLLITCLLT ALEAVPIDID KTKVQNIHPV ESAKIEPPDT GLYYDEYLKQ 60
VIDVLETDKH FREKLQKADI EEIKSGRLSK ELDLVSHHVR TKLDELKRQE VGRLRMLIKA 120
KLDSLQDIGM DHQALLKQFD HLNHLNPDKF ESTDLDMLIK AATSDLEHYD KTRHEEFKKY 180
EMMKEHERRE YLKTLNEEKR KEEESKFEEM KKKHENHPKV NHPGSKDQLK EVWEETDGLD 240
PNDFDPKTFF KLHDVNSDGF LDEQELEALF TKELEKVYDP KNEEDDMVEM EEERLRMREH 300
VMSEVDTNKD RLVTLEEFLK ATEKKEFLEP DSWETLDQQQ FFTEEELKEY ENIIALQENE 360
LKKKADELQK QKEELQRQHD QLEAQKLEYH QVIQQMEQKK LQQGIPPSGP AGELKFEPHI 420 
Gene Ontology
 GO:0005829; C:cytosol; TAS:ProtInc.
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IDA:UniProtKB.
 GO:0005615; C:extracellular space; TAS:ProtInc.
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0005640; C:nuclear outer membrane; IEA:Compara.
 GO:0005886; C:plasma membrane; TAS:ProtInc.
 GO:0005509; F:calcium ion binding; TAS:ProtInc.
 GO:0003677; F:DNA binding; TAS:ProtInc. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom. 
Pfam
 PF13499; EF_hand_5 
SMART
 SM00054; EFh 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 
PRINTS