CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018069
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Short transient receptor potential channel 4-associated protein 
Protein Synonyms/Alias
 Trp4-associated protein; Trpc4-associated protein; Protein TAP1; TNF-receptor ubiquitous scaffolding/signaling protein; Protein TRUSS 
Gene Name
 TRPC4AP 
Gene Synonyms/Alias
 C20orf188; TRRP4AP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
70TERDKQSKWSGIPQLubiquitination[1, 2, 3]
157SIRGQKLKISDEMSKubiquitination[4]
182VCTEGVTKRLAEKNDubiquitination[2]
218IEDILGVKKEMIRLDubiquitination[2, 4]
219EDILGVKKEMIRLDEubiquitination[2]
263MDTGNDDKHTLLAKNubiquitination[2]
269DKHTLLAKNAQQKKSubiquitination[2, 4]
275AKNAQQKKSLSLGPSubiquitination[1, 2, 3, 4]
303GFVERLCKLATRKVSubiquitination[2, 4]
415HRMIAEFKLIPGLNNubiquitination[2, 4]
472FSDHHENKYLLLNNQubiquitination[1, 2, 3, 4]
511RSLVCDGKRGLLTRLubiquitination[2, 5]
523TRLLQVMKKEPAESSubiquitination[1, 2, 3]
524RLLQVMKKEPAESSFubiquitination[2]
557ADQMFLLKRGLLEHIubiquitination[1, 2, 3, 4]
598KFNVDAFKRFNKYINubiquitination[1, 3, 4]
602DAFKRFNKYINTDAKubiquitination[2, 4]
720QRMEHSKKYPGFLLNubiquitination[1, 2, 3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC. Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca(2+) storage reduction in response to CHRM1. 
Sequence Annotation
 REGION 1 400 Interaction with TNFRSF1A (By  
Keyword
 Alternative splicing; Complete proteome; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 797 AA 
Protein Sequence
MAAAPVAAGS GAGRGRRSAA TVAAWGGWGG RPRPGNILLQ LRQGQLTGRG LVRAVQFTET 60
FLTERDKQSK WSGIPQLLLK LHTTSHLHSD FVECQNILKE ISPLLSMEAM AFVTEERKLT 120
QETTYPNTYI FDLFGGVDLL VEILMRPTIS IRGQKLKISD EMSKDCLSIL YNTCVCTEGV 180
TKRLAEKNDF VIFLFTLMTS KKTFLQTATL IEDILGVKKE MIRLDEVPNL SSLVSNFDQQ 240
QLANFCRILA VTISEMDTGN DDKHTLLAKN AQQKKSLSLG PSAAEINQAA LLSIPGFVER 300
LCKLATRKVS ESTGTASFLQ ELEEWYTWLD NALVLDALMR VANEESEHNQ ASIVFPPPGA 360
SEENGLPHTS ARTQLPQSMK IMHEIMYKLE VLYVLCVLLM GRQRNQVHRM IAEFKLIPGL 420
NNLFDKLIWR KHSASALVLH GHNQNCDCSP DITLKIQFLR LLQSFSDHHE NKYLLLNNQE 480
LNELSAISLK ANIPEVEAVL NTDRSLVCDG KRGLLTRLLQ VMKKEPAESS FRFWQARAVE 540
SFLRGTTSYA DQMFLLKRGL LEHILYCIVD SECKSRDVLQ SYFDLLGELM KFNVDAFKRF 600
NKYINTDAKF QVFLKQINSS LVDSNMLVRC VTLSLDRFEN QVDMKVAEVL SECRLLAYIS 660
QVPTQMSFLF RLINIIHVQT LTQENVSCLN TSLVILMLAR RKERLPLYLR LLQRMEHSKK 720
YPGFLLNNFH NLLRFWQQHY LHKDKDSTCL ENSSCISFSY WKETVSILLN PDRQSPSALV 780
SYIEEPYMDI DRDFTEE 797 
Gene Ontology
 GO:0031464; C:Cul4A-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0019902; F:phosphatase binding; IDA:UniProtKB.
 GO:0016567; P:protein ubiquitination; IDA:UniProtKB.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IDA:UniProtKB. 
Interpro
 IPR022162; DUF3689. 
Pfam
 PF12463; DUF3689 
SMART
  
PROSITE
  
PRINTS