CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032892
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 H/ACA ribonucleoprotein complex subunit 4 
Protein Synonyms/Alias
  
Gene Name
 DKC1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
12EVIILPKKHKKKKERubiquitination[1]
20HKKKKERKSLPEEDVubiquitination[1, 2]
39HAEEFLIKPESKVAKubiquitination[1, 2, 3, 4, 5]
43FLIKPESKVAKLDTSubiquitination[3]
46KPESKVAKLDTSQWPubiquitination[1, 2, 4, 5]
57SQWPLLLKNFDKLNVubiquitination[1, 2, 3, 4]
61LLLKNFDKLNVRTTHubiquitination[1, 5]
80ACGSNPLKREIGDYIubiquitination[1, 3, 5]
96TGFINLDKPSNPSSHubiquitination[1]
117RRILRVEKTGHSGTLubiquitination[1]
151KSQQSAGKEYVGIVRubiquitination[1, 2, 5]
191PPLIAAVKRQLRVRTubiquitination[1, 3, 4, 5]
203VRTIYESKMIEYDPEubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 259 AA 
Protein Sequence
MADAEVIILP KKHKKKKERK SLPEEDVAEI QHAEEFLIKP ESKVAKLDTS QWPLLLKNFD 60
KLNVRTTHYT PLACGSNPLK REIGDYIRTG FINLDKPSNP SSHEVVAWIR RILRVEKTGH 120
SGTLDPKVTG CLIVCIERAT RLVKSQQSAG KEYVGIVRLH NAIEGGTQLS RALETLTGAL 180
FQRPPLIAAV KRQLRVRTIY ESKMIEYDPE RRLGIFWDHM VTMHDVLDAQ WLYDNHKDES 240
YLRRVVYPLE KLLTSHKRL 259 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
 GO:0006396; P:RNA processing; IEA:InterPro. 
Interpro
 IPR012960; Dyskerin-like.
 IPR002501; PsdUridine_synth.
 IPR020103; PsdUridine_synth_cat_dom.
 IPR004802; tRNA_PsdUridine_synth_B_fam. 
Pfam
 PF08068; DKCLD
 PF01509; TruB_N 
SMART
  
PROSITE
  
PRINTS