UniProt Accession

 DHRS4_MOUSE; Q99LB2; G3X8V7; Q9EQU4 

Genbank Protein ID

 AB045132; AC159002; CH466535; BC003484; BC054361 

Genbank Nucleotide ID

 BAB18776.1; EDL36299.1; AAH03484.1; AAH54361.1 

Protein Name

 Dehydrogenase/reductase SDR family member 4 

Protein Synonyms/Alias

 NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; CR; PHCR; NADPH-dependent retinol dehydrogenase/reductase; NDRD; mouNRDR; Peroxisomal short-chain alcohol dehydrogenase; PSCD 

Gene Name

 Dhrs4 

Gene Synonyms/Alias

 D14Ucla2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
93	GIVCHVGKAEDREKL	acetylation	[1, 2, 3, 4, 5]
93	GIVCHVGKAEDREKL	succinylation	[4]
99	GKAEDREKLITTALK	acetylation	[2, 3, 5]
106	KLITTALKRHQGIDI	acetylation	[3, 4, 5]
106	KLITTALKRHQGIDI	succinylation	[4]
106	KLITTALKRHQGIDI	ubiquitination	[6]
159	AVVPEMEKRGGGSVV	acetylation	[2, 3, 5]
187	LGPYNVSKTALLGLT	ubiquitination	[6]
195	TALLGLTKNFAAELA	ubiquitination	[6]
204	FAAELAPKNIRVNCL	acetylation	[3, 5, 7]
217	CLAPGLIKTRFSSVL	acetylation	[2, 3, 4, 5, 7, 8]
217	CLAPGLIKTRFSSVL	succinylation	[4]
217	CLAPGLIKTRFSSVL	ubiquitination	[6]
228	SSVLWEEKAREDFIK	acetylation	[2, 3, 4, 5, 8]
228	SSVLWEEKAREDFIK	succinylation	[4]
235	KAREDFIKEAMQIRR	acetylation	[4]
235	KAREDFIKEAMQIRR	succinylation	[4]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [6] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [7] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [8] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199] 

Functional Description

 Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co- factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones (By similarity). 

Sequence Annotation

 NP_BIND 37 61 NADP (By similarity).
 MOTIF 277 279 Microbody targeting signal.
 ACT_SITE 183 183 Proton acceptor (By similarity).
 BINDING 170 170 Substrate (By similarity).  

Keyword

 Complete proteome; NADP; Oxidoreductase; Peroxisome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 279 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0005777; C:peroxisome; IDA:MGI.
 GO:0004090; F:carbonyl reductase (NADPH) activity; IEA:EC.
 GO:0001758; F:retinal dehydrogenase activity; IDA:MGI.
 GO:0042574; P:retinal metabolic process; IDA:MGI. 

Interpro

 IPR002198; DH_sc/Rdtase_SDR.
 IPR027052; DHRS2/4.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 

Pfam

  

SMART

  

PROSITE

 PS00061; ADH_SHORT 

PRINTS

 PR00081; GDHRDH.
 PR00080; SDRFAMILY.