UniProt Accession

 TM1L1_HUMAN; O75674; Q53G06 

Genbank Protein ID

 AJ010071; AK315039; AK223125; CH471109 

Genbank Nucleotide ID

 CAA08993.1; BAG37522.1; BAD96845.1; EAW94553.1 

Protein Name

 TOM1-like protein 1 

Protein Synonyms/Alias

 Src-activating and signaling molecule protein; Target of Myb-like protein 1 

Gene Name

 TOM1L1 

Gene Synonyms/Alias

 SRCASM 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MAFGKSHRDPYA	ubiquitination	[1, 2, 3]
103	FVKENLVKLLNPRYN	ubiquitination	[1, 3]
148	EVYLDLVKKGVQFPP	ubiquitination	[1, 3, 4, 5]
191	LSSVIAPKNSTVTLV	ubiquitination	[1, 3, 4, 5]
298	QRILEQNKNQKEATN	ubiquitination	[4, 5, 6]
301	LEQNKNQKEATNTTS	ubiquitination	[4, 5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

 Probable adapter protein involved in signaling pathways. Interacts with the SH2 and SH3 domains of various signaling proteins when it is phosphorylated. May promote FYN activation, possibly by disrupting intramolecular SH3-dependent interactions (By similarity). 

Sequence Annotation

 DOMAIN 22 154 VHS.
 DOMAIN 200 288 GAT.
 REGION 392 395 Interaction with GRB2 (By similarity).
 REGION 442 445 Interaction with PIK3R1 (By similarity).
 MOTIF 421 425 SH3-binding (By similarity).
 MOTIF 460 463 SH2-binding (By similarity).
 MOD_RES 314 314 Phosphoserine.
 MOD_RES 321 321 Phosphoserine.
 MOD_RES 323 323 Phosphoserine.
 MOD_RES 460 460 Phosphotyrosine (By similarity).  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Endosome; Golgi apparatus; Membrane; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; SH3-binding; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 476 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:HGNC.
 GO:0005768; C:endosome; IDA:HGNC.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005795; C:Golgi stack; IEA:UniProtKB-SubCell.
 GO:0005764; C:lysosome; TAS:ProtInc.
 GO:0030295; F:protein kinase activator activity; IEA:Compara.
 GO:0043130; F:ubiquitin binding; TAS:HGNC.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; NAS:HGNC. 

Interpro

 IPR008942; ENTH_VHS.
 IPR004152; GAT.
 IPR014645; TOM1.
 IPR027428; TOM1L1.
 IPR002014; VHS.
 IPR018205; VHS_subgr. 

Pfam

 PF03127; GAT
 PF00790; VHS 

SMART

 SM00288; VHS 

PROSITE

 PS50909; GAT
 PS50179; VHS 

PRINTS