UniProt Accession

 UBC13_YEAST; P52490; D6VS79 

Genbank Protein ID

 X99443; Z50111; BK006938 

Genbank Nucleotide ID

 CAA67806.1; CAA90451.1; DAA11939.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 13 

Protein Synonyms/Alias

 Ubiquitin carrier protein 13; Ubiquitin-protein ligase 13 

Gene Name

 UBC13 

Gene Synonyms/Alias

 YDR092W; YD6652.04 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
14	RIIKETEKLVSDPVP	acetylation	[1]
74	PKVRFLTKIYHPNID	acetylation	[1]
74	PKVRFLTKIYHPNID	ubiquitination	[2]
92	RICLDVLKTNWSPAL	ubiquitination	[2, 3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Has a role in the DNA error-free postreplication repair (PRR) pathway. The UBC13/MMS2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. 

Sequence Annotation

 ACT_SITE 87 87 Glycyl thioester intermediate.  

Keyword

 3D-structure; ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 153 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0031371; C:ubiquitin conjugating enzyme complex; IPI:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:EC.
 GO:0010994; P:free ubiquitin chain polymerization; IDA:SGD.
 GO:0006301; P:postreplication repair; IMP:SGD.
 GO:0006513; P:protein monoubiquitination; TAS:SGD.
 GO:0000209; P:protein polyubiquitination; IDA:SGD. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS