CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018001
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Signal peptide peptidase-like 2B 
Protein Synonyms/Alias
 SPP-like 2B; SPPL2b; Intramembrane protease 4; IMP-4; Presenilin homologous protein 4; PSH4; Presenilin-like protein 1 
Gene Name
 SPPL2B 
Gene Synonyms/Alias
 IMP4; KIAA1532; PSL1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
103GNCTFYEKVRLAQGSubiquitination[1]
398EKLPMVLKVPRLNSSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF- alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. May play a role in the regulation of innate and adaptive immunity. 
Sequence Annotation
 DOMAIN 71 149 PA.
 MOTIF 472 474 PAL.
 ACT_SITE 359 359 By similarity.
 ACT_SITE 421 421 By similarity.
 CARBOHYD 97 97 N-linked (GlcNAc...) (Potential).
 CARBOHYD 129 129 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Complete proteome; Glycoprotein; Golgi apparatus; Hydrolase; Membrane; Polymorphism; Protease; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 592 AA 
Protein Sequence
MAAAVAAALA RLLAAFLLLA AQVACEYGMV HVVSQAGGPE GKDYCILYNP QWAHLPHDLS 60
KASFLQLRNW TASLLCSAAD LPARGFSNQI PLVARGNCTF YEKVRLAQGS GARGLLIVSR 120
ERLVPPGGNK TQYDEIGIPV ALLSYKDMLD IFTRFGRTVR AALYAPKEPV LDYNMVIIFI 180
MAVGTVAIGG YWAGSRDVKK RYMKHKRDDG PEKQEDEAVD VTPVMTCVFV VMCCSMLVLL 240
YYFYDLLVYV VIGIFCLASA TGLYSCLAPC VRRLPFGKCR IPNNSLPYFH KRPQARMLLL 300
ALFCVAVSVV WGVFRNEDQW AWVLQDALGI AFCLYMLKTI RLPTFKACTL LLLVLFLYDI 360
FFVFITPFLT KSGSSIMVEV ATGPSDSATR EKLPMVLKVP RLNSSPLALC DRPFSLLGFG 420
DILVPGLLVA YCHRFDIQVQ SSRVYFVACT IAYGVGLLVT FVALALMQRG QPALLYLVPC 480
TLVTSCAVAL WRRELGVFWT GSGFAKVLPP SPWAPAPADG PQPPKDSATP LSPQPPSEEP 540
ATSPWPAEQS PKSRTSEEMG AGAPMREPGS PAESEGRDQA QPSPVTQPGA SA 592 
Gene Ontology
 GO:0010008; C:endosome membrane; IDA:UniProtKB.
 GO:0030660; C:Golgi-associated vesicle membrane; IDA:UniProtKB.
 GO:0071458; C:integral to cytosolic side of endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0071556; C:integral to lumenal side of endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0005765; C:lysosomal membrane; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0042500; F:aspartic endopeptidase activity, intramembrane cleaving; IMP:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0006509; P:membrane protein ectodomain proteolysis; IDA:UniProtKB.
 GO:0031293; P:membrane protein intracellular domain proteolysis; IDA:UniProtKB.
 GO:0050776; P:regulation of immune response; IMP:UniProtKB. 
Interpro
 IPR006639; Peptidase_A22.
 IPR007369; Peptidase_A22B_SPP.
 IPR003137; Protease-assoc_domain. 
Pfam
 PF02225; PA
 PF04258; Peptidase_A22B 
SMART
 SM00730; PSN 
PROSITE
  
PRINTS