CPLM-001270

Genbank Nucleotide ID

26S proteasome non-ATPase regulatory subunit 10

Protein Synonyms/Alias

26S proteasome regulatory subunit p28; Gankyrin; p28(GANK)

Homo sapiens (Human)

Position	Peptide	Type	References
23	SGKLEELKESILADK	ubiquitination	[1, 2, 3, 4]
30	KESILADKSLATRTD	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8]
90	AGRDEIVKALLGKGA	ubiquitination	[1, 4, 7]
153	AAAKGNLKMIHILLY	ubiquitination	[4, 5]
213	KTPLQVAKGGLGLIL	ubiquitination	[1, 4]
221	GGLGLILKRMVEG**	ubiquitination	[1, 4]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[5] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.

REPEAT 3 36 ANK 1.
REPEAT 37 69 ANK 2.
REPEAT 70 102 ANK 3.
REPEAT 103 135 ANK 4.
REPEAT 136 168 ANK 5.
REPEAT 169 201 ANK 6.
REPEAT 202 226 ANK 7.
REGION 1 71 Interaction with RB1.
REGION 1 37 Required for nuclear localization.
REGION 39 226 Interaction with RELA.
REGION 171 226 Interaction with RB1.

3D-structure; Alternative splicing; ANK repeat; Apoptosis; Chaperone; Complete proteome; Cytoplasm; Nucleus; Reference proteome; Repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0015629; C:actin cytoskeleton; IDA:HPA.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0045111; C:intermediate filament cytoskeleton; IDA:HPA.
GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0005838; C:proteasome regulatory particle; TAS:ProtInc.
GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; TAS:Reactome.
GO:0002479; P:antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; TAS:Reactome.
GO:0006915; P:apoptotic process; TAS:Reactome.
GO:0007253; P:cytoplasmic sequestering of NF-kappaB; IDA:UniProtKB.
GO:0006977; P:DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; TAS:Reactome.
GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome.
GO:0010467; P:gene expression; TAS:Reactome.
GO:0016071; P:mRNA metabolic process; TAS:Reactome.
GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
GO:0043518; P:negative regulation of DNA damage response, signal transduction by p53 class mediator; IDA:UniProtKB.
GO:0043409; P:negative regulation of MAPK cascade; IMP:UniProtKB.
GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IDA:UniProtKB.
GO:0090201; P:negative regulation of release of cytochrome c from mitochondria; IMP:UniProtKB.
GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
GO:0051436; P:negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
GO:0030307; P:positive regulation of cell growth; IDA:UniProtKB.
GO:0045737; P:positive regulation of cyclin-dependent protein kinase activity; IDA:UniProtKB.
GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
GO:0051437; P:positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; TAS:Reactome.
GO:0070682; P:proteasome regulatory particle assembly; IMP:UniProtKB.
GO:0000209; P:protein polyubiquitination; TAS:Reactome.
GO:0006521; P:regulation of cellular amino acid metabolic process; TAS:Reactome.
GO:0044281; P:small molecule metabolic process; TAS:Reactome.
GO:0016032; P:viral reproduction; TAS:Reactome.

IPR002110; Ankyrin_rpt.
IPR020683; Ankyrin_rpt-contain_dom.

PS50297; ANK_REP_REGION
PS50088; ANK_REPEAT