CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015251
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lysine-specific demethylase 3A 
Protein Synonyms/Alias
 JmjC domain-containing histone demethylation protein 2A; Jumonji domain-containing protein 1A 
Gene Name
 Kdm3a 
Gene Synonyms/Alias
 Jhdm2a; Jmjd1a; Kiaa0742 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
895LLNSSTAKTENGLKNubiquitination[1]
918FASLVQNKTSSDSSKubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation (By similarity). Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. 
Sequence Annotation
 DOMAIN 1060 1283 JmjC.
 ZN_FING 662 687 C6-type (Potential).
 MOTIF 885 889 LXXLL motif.
 METAL 1122 1122 Iron; catalytic (By similarity).
 METAL 1124 1124 Iron; catalytic (By similarity).
 METAL 1251 1251 Iron; catalytic (By similarity).
 MOD_RES 264 264 Phosphoserine (By similarity).
 MOD_RES 895 895 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Activator; Alternative splicing; Chromatin regulator; Complete proteome; Cytoplasm; Differentiation; Dioxygenase; Iron; Metal-binding; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome; Spermatogenesis; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1323 AA 
Protein Sequence
MVLTLGESWP VLVGKRFLSL SAAEGNEGGQ DNWDLERVAE WPWLSGTIRA VSHTDVTKKD 60
LKVCVEFDGE SWRKRRWIDV YSLQRKAFLV EHNLVLAERK SPEVPEQVIQ WPAIMYKSLL 120
DKAGLGAITS VRFLGDQQSV FVSKDLLKPI QDVNSLRLSL TDNQTVSKEF QALIVKHLDE 180
SHLLQGDKNL VGSEVKIYSL DPSTQWFSAT VVHGNPSSKT LQVNCEEIPA LKIVDPALIH 240
VEVVHDNFVT CGNSTRTGAV KRKSSENNGS SVSKQAKSCS EASPSMCPVQ SVPTTVFKEI 300
LLGCTAATPS SKDPRQQNTP QAANSPPNIG AKLPQGCHKQ NLPEELSSCL NTKPEVPRTK 360
PDVCKEGLLS SKSSQVGAGD LKILSEPKGS CIQPKTNTDQ ESRLESAPQP VTGLPKECLP 420
AKTSSKAELD IATTPELQKH LEHAASTSDD LSDKPEVKAG VTSLNSCAEK KVEPSHLGSQ 480
SQNLKETSVK VDNESCCTRS SNKTQTPPAR KSVLTDPDKV RKLQQSGEAF VQDDSCVNIV 540
AQLPKCRECR LDSLRKDKDQ QKDSPVFCRF FHFRRLQFNK HGVLRVEGFL TPNKYDSEAI 600
GLWLPLTKNV VGTDLDTAKY ILANIGDHFC QMVISEKEAM STIEPHRQVA WKRAVKGVRE 660
MCDVCDTTIF NLHWVCPRCG FGVCVDCYRM KRKNCQQGAA YKTFSWIRCV KSQIHEPENL 720
MPTQIIPGKA LYDVGDIVHS VRAKWGIKAN CPCSNRQFKL FSKPALKEDL KQTSLSGEKP 780
TLGTMVQQSS PVLEPVAVCG EAASKPASSV KPTCPTSTSP LNWLADLTSG NVNKENKEKQ 840
LTMPILKNEI KCLPPLPPLN KPSTVLHTFN STILTPVSNN NSGFLRNLLN SSTAKTENGL 900
KNTPKILDDI FASLVQNKTS SDSSKRPQGL TIKPSILGFD TPHYWLCDNR LLCLQDPNNK 960
SNWNVFRECW KQGQPVMVSG VHHKLNTELW KPESFRKEFG EQEVDLVNCR TNEIITGATV 1020
GDFWDGFEDV PNRLKNDKEK EPMVLKLKDW PPGEDFRDMM PSRFDDLMAN IPLPEYTRRD 1080
GKLNLASRLP NYFVRPDLGP KMYNAYGLIT PEDRKYGTTN LHLDVSDAAN VMVYVGIPKG 1140
QCEQEEEVLR TIQDGDSDEL TIKRFIEGKE KPGALWHIYA AKDTEKIREF LKKVSEEQGQ 1200
DNPADHDPIH DQSWYLDRSL RKRLYQEYGV QGWAIVQFLG DVVFIPAGAP HQVHNLYSCI 1260
KVAEDFVSPE HVKHCFWLTQ EFRYLSQTHT NHEDKLQVKN VIYHAVKDAV AMLKASESSL 1320
GKP 1323 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0001047; F:core promoter binding; IDA:MGI.
 GO:0005506; F:iron ion binding; IEA:Compara.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:MGI.
 GO:0000976; F:transcription regulatory region sequence-specific DNA binding; IDA:MGI.
 GO:0030521; P:androgen receptor signaling pathway; IEA:Compara.
 GO:0046293; P:formaldehyde biosynthetic process; IEA:Compara.
 GO:0033169; P:histone H3-K9 demethylation; IEA:Compara.
 GO:0036123; P:histone H3-K9 dimethylation; IMP:MGI.
 GO:0009755; P:hormone-mediated signaling pathway; IEA:Compara.
 GO:0051573; P:negative regulation of histone H3-K9 methylation; IMP:MGI.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0007290; P:spermatid nucleus elongation; IMP:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR003347; JmjC_dom. 
Pfam
 PF02373; JmjC 
SMART
 SM00558; JmjC 
PROSITE
 PS51184; JMJC 
PRINTS