UniProt Accession

 UBE2N_MOUSE; P61089; Q16781; Q3TSL6; Q6ZWZ0; Q9DAJ6 

Genbank Protein ID

 Y09873; AY039837; AK005302; AK005788; AK161968; BC034898; BC067069 

Genbank Nucleotide ID

 CAA71001.1; AAK74128.1; BAB23941.1; BAB24239.1; BAE36659.1; AAH34898.3; AAH67069.1 

Protein Name

 Ubiquitin-conjugating enzyme E2 N 

Protein Synonyms/Alias

 Bendless-like ubiquitin-conjugating enzyme; Ubc13; Ubiquitin carrier protein N; Ubiquitin-protein ligase N 

Gene Name

 Ube2n 

Gene Synonyms/Alias

 Blu 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
10	GLPRRIIKETQRLLA	acetylation	[1]
10	GLPRRIIKETQRLLA	ubiquitination	[2]
24	AEPVPGIKAEPDESN	acetylation	[1, 3]
24	AEPVPGIKAEPDESN	ubiquitination	[2]
68	EYPMAAPKVRFMTKI	ubiquitination	[2]
74	PKVRFMTKIYHPNVD	acetylation	[1, 3]
74	PKVRFMTKIYHPNVD	ubiquitination	[2]
82	IYHPNVDKLGRICLD	acetylation	[1, 3]
82	IYHPNVDKLGRICLD	ubiquitination	[2]
92	RICLDILKDKWSPAL	ubiquitination	[2]
94	CLDILKDKWSPALQI	acetylation	[1, 4]
94	CLDILKDKWSPALQI	succinylation	[4]
94	CLDILKDKWSPALQI	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD (By similarity). Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes (By similarity). 

Sequence Annotation

 ACT_SITE 87 87 Glycyl thioester intermediate.
 MOD_RES 82 82 N6-acetyllysine (By similarity).
 CROSSLNK 92 92 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; DNA damage; DNA repair; Isopeptide bond; Ligase; Nucleotide-binding; Nucleus; Reference proteome; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 152 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0031372; C:UBC13-MMS2 complex; ISS:HGNC.
 GO:0035370; C:UBC13-UEV1A complex; ISS:UniProtKB.
 GO:0000151; C:ubiquitin ligase complex; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0043130; F:ubiquitin binding; ISS:HGNC.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0000729; P:DNA double-strand break processing; ISS:HGNC.
 GO:0000724; P:double-strand break repair via homologous recombination; ISS:HGNC.
 GO:0016574; P:histone ubiquitination; ISS:HGNC.
 GO:0045739; P:positive regulation of DNA repair; ISS:HGNC.
 GO:0031058; P:positive regulation of histone modification; ISS:HGNC.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; ISS:HGNC.
 GO:0051092; P:positive regulation of NF-kappaB transcription factor activity; ISS:HGNC.
 GO:0051443; P:positive regulation of ubiquitin-protein ligase activity; ISS:HGNC.
 GO:0006301; P:postreplication repair; ISS:HGNC.
 GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
 GO:0033182; P:regulation of histone ubiquitination; ISS:HGNC.
 GO:0050852; P:T cell receptor signaling pathway; ISS:HGNC.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IDA:MGI. 

Interpro

 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS