UniProt Accession

 RUVA_ECOLI; P0A809; P08576 

Genbank Protein ID

 X07091; M21298; U00096; AP009048; D10165 

Genbank Nucleotide ID

 CAA30119.1; AAA24612.1; AAC74931.1; BAA15672.1; BAA01034.1 

Protein Name

 Holliday junction ATP-dependent DNA helicase RuvA 

Protein Synonyms/Alias

  

Gene Name

 ruvA 

Gene Synonyms/Alias

 b1861; JW1850 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
66	LLYGFNNKQERTLFK	acetylation	[1]
73	KQERTLFKELIKTNG	acetylation	[1]
77	TLFKELIKTNGVGPK	acetylation	[1]
129	ERLIVEMKDRFKGLH	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA. 

Sequence Annotation

  

Keyword

 3D-structure; ATP-binding; Complete proteome; Cytoplasm; DNA damage; DNA recombination; DNA repair; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome; SOS response. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 203 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0009379; C:Holliday junction helicase complex; IDA:EcoCyc.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0009378; F:four-way junction helicase activity; IDA:EcoCyc.
 GO:0000725; P:recombinational repair; IMP:EcoCyc.
 GO:0009432; P:SOS response; IEP:EcoCyc. 

Interpro

 IPR011114; DNA_helicas_Holl-junc_RuvA_C.
 IPR013849; DNA_helicase_Holl-junc_RuvA_I.
 IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
 IPR012340; NA-bd_OB-fold.
 IPR000085; RuvA.
 IPR010994; RuvA_2-like. 

Pfam

 PF07499; RuvA_C
 PF01330; RuvA_N 

SMART

 SM00278; HhH1 

PROSITE

  

PRINTS