CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010963
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Interferon regulatory factor 8 
Protein Synonyms/Alias
 IRF-8; Interferon consensus sequence-binding protein; H-ICSBP; ICSBP 
Gene Name
 IRF8 
Gene Synonyms/Alias
 ICSBP1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
240QPGLPGTKLYGPEGLubiquitination[1, 2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity). 
Sequence Annotation
 DNA_BIND 7 114 IRF tryptophan pentad repeat.  
Keyword
 Complete proteome; DNA-binding; Nucleus; Polymorphism; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 426 AA 
Protein Sequence
MCDRNGGRRL RQWLIEQIDS SMYPGLIWEN EEKSMFRIPW KHAGKQDYNQ EVDASIFKAW 60
AVFKGKFKEG DKAEPATWKT RLRCALNKSP DFEEVTDRSQ LDISEPYKVY RIVPEEEQKC 120
KLGVATAGCV NEVTEMECGR SEIDELIKEP SVDDYMGMIK RSPSPPEACR SQLLPDWWAQ 180
QPSTGVPLVT GYTTYDAHHS AFSQMVISFY YGGKLVGQAT TTCPEGCRLS LSQPGLPGTK 240
LYGPEGLELV RFPPADAIPS ERQRQVTRKL FGHLERGVLL HSSRQGVFVK RLCQGRVFCS 300
GNAVVCKGRP NKLERDEVVQ VFDTSQFFRE LQQFYNSQGR LPDGRVVLCF GEEFPDMAPL 360
RSKLILVQIE QLYVRQLAEE AGKSCGAGSV MQAPEEPPPD QVFRMFPDIC ASHQRSFFRE 420
NQQITV 426 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0000975; F:regulatory region DNA binding; IEA:InterPro.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0071222; P:cellular response to lipopolysaccharide; IEA:Compara.
 GO:0042742; P:defense response to bacterium; IEA:Compara.
 GO:0042832; P:defense response to protozoan; IEA:Compara.
 GO:0060333; P:interferon-gamma-mediated signaling pathway; TAS:Reactome.
 GO:0030099; P:myeloid cell differentiation; IEA:Compara.
 GO:0044130; P:negative regulation of growth of symbiont in host; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0006909; P:phagocytosis; IEA:Compara.
 GO:0032729; P:positive regulation of interferon-gamma production; IEA:Compara.
 GO:0032735; P:positive regulation of interleukin-12 production; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0060337; P:type I interferon-mediated signaling pathway; TAS:Reactome. 
Interpro
 IPR019817; Interferon_reg_fac_CS.
 IPR001346; Interferon_reg_fact_DNA-bd_dom.
 IPR019471; Interferon_reg_factor-3.
 IPR017855; SMAD_dom-like.
 IPR008984; SMAD_FHA_domain.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00605; IRF
 PF10401; IRF-3 
SMART
 SM00348; IRF 
PROSITE
 PS00601; IRF_1
 PS51507; IRF_2 
PRINTS
 PR00267; INTFRNREGFCT.