UniProt Accession

 GLMU_ECOLI; P0ACC7; P17114; P76746; Q2M848 

Genbank Protein ID

 X01631; L10328; L10328; U00096; AP009048 

Genbank Nucleotide ID

 CAA25784.1; AAA62082.1; AAA62081.1; AAC76753.1; BAE77558.1 

Protein Name

 Bifunctional protein GlmU 

Protein Synonyms/Alias

 UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase 

Gene Name

 glmU 

Gene Synonyms/Alias

 yieA; b3730; JW3708 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
25	RMYSDLPKVLHTLAG	acetylation	[1]
63	GHGGDLLKQALKDDN	acetylation	[1]
132	GIGLLTVKLDDPTGY	acetylation	[1]
156	VTGIVEHKDATDEQR	acetylation	[1]
246	YQSEQAEKLLLAGVM	acetylation	[1]
351	VGNFVEMKKARLGKG	acetylation	[1, 2]
360	ARLGKGSKAGHLTYL	acetylation	[1]
392	CNYDGANKFKTIIGD	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP- GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5- monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain. 

Sequence Annotation

 REGION 1 229 Pyrophosphorylase.
 REGION 11 14 UDP-GlcNAc binding.
 REGION 81 82 UDP-GlcNAc binding.
 REGION 103 105 UDP-GlcNAc binding.
 REGION 230 250 Linker.
 REGION 251 456 N-acetyltransferase.
 REGION 386 387 Acetyl-CoA binding.
 ACT_SITE 363 363 Proton acceptor (By similarity).
 METAL 105 105 Magnesium.
 METAL 227 227 Magnesium.
 BINDING 25 25 UDP-GlcNAc (By similarity).
 BINDING 76 76 UDP-GlcNAc.
 BINDING 140 140 UDP-GlcNAc; via amide nitrogen.
 BINDING 154 154 UDP-GlcNAc.
 BINDING 169 169 UDP-GlcNAc.
 BINDING 227 227 UDP-GlcNAc (By similarity).
 BINDING 333 333 UDP-GlcNAc.
 BINDING 351 351 UDP-GlcNAc.
 BINDING 366 366 UDP-GlcNAc.
 BINDING 377 377 UDP-GlcNAc.
 BINDING 380 380 Acetyl-CoA; via amide nitrogen.
 BINDING 405 405 Acetyl-CoA.
 BINDING 423 423 Acetyl-CoA; via amide nitrogen.
 BINDING 440 440 Acetyl-CoA.  

Keyword

 3D-structure; Acyltransferase; Cell shape; Cell wall biogenesis/degradation; Cobalt; Complete proteome; Cytoplasm; Magnesium; Metal-binding; Multifunctional enzyme; Nucleotidyltransferase; Peptidoglycan synthesis; Reference proteome; Repeat; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 456 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0019134; F:glucosamine-1-phosphate N-acetyltransferase activity; IDA:EcoCyc.
 GO:0042802; F:identical protein binding; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IDA:EcoCyc.
 GO:0003977; F:UDP-N-acetylglucosamine diphosphorylase activity; IDA:EcoCyc.
 GO:0000902; P:cell morphogenesis; IEA:HAMAP.
 GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:InterPro.
 GO:0009252; P:peptidoglycan biosynthetic process; IEA:HAMAP.
 GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
 GO:0006048; P:UDP-N-acetylglucosamine biosynthetic process; IMP:EcoCyc. 

Interpro

 IPR005882; Bifunctional_GlmU.
 IPR001451; Hexapep_transf.
 IPR018357; Hexapep_transf_CS.
 IPR025877; MobA-like_NTP_Trfase_dom.
 IPR011004; Trimer_LpxA-like. 

Pfam

 PF00132; Hexapep
 PF12804; NTP_transf_3 

SMART

  

PROSITE

 PS00101; HEXAPEP_TRANSFERASES 

PRINTS