CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040674
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 
Protein Synonyms/Alias
  
Gene Name
 AGAP2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
540RALCADMKRCSYYETubiquitination[1]
565VFQEVAQKVVTLRKQubiquitination[1]
571QKVVTLRKQQQLLAAubiquitination[1]
1037RESWIRAKYEQLLFLubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 ANK repeat; Complete proteome; Metal-binding; Reference proteome; Repeat; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1192 AA 
Protein Sequence
MSRGAGALQR RTTTYLISLT LVKLESVPPP PPSPSAAAAG AAGARGSETG DPGSPRGAEE 60
PGKKRHERLF HRQDALWIST SSAGTGGAEP PALSPAPASP ARPVSPAPGR RLSLWAVPPG 120
PPLSGGLSPD PKPGGAPTSS RRPLLSSPSW GGPEPEGRAG GGIPGSSSPH PGTGSRRLKV 180
APPPPAPKPC KTVTTSGAKA GGGKGAGSRL SWPESEGKPR VKGSKSSAGT GASVSAAATA 240
AAAGGGGSTA STSGGVGAGA GARGKLSPRK GKSKTLDNSD LHPGPPAGSP PPLTLPPTPS 300
PATAVTAASA QPPGPAPPIT LEPPAPGLKR GREGGRASTR DRKMLKFISG IFTKSTGGPP 360
GSGPLPGPPS LSSGSGSREL LGAELRASPK AVINSQEWTL SRSIPELRLG VLGDARSGKS 420
SLIHRFLTGS YQVLEKTESE QYKKEMLVDG QTHLVLIREE AGAPDAKFSG WADAVIFVFS 480
LEDENSFQAV SRLHGQLSSL RGEGRGGLAL ALVGTQDRIS ASSPRVVGDA RARALCADMK 540
RCSYYETCAT YGLNVDRVFQ EVAQKVVTLR KQQQLLAACK SLPSSPSHSA ASTPVAGQAS 600
NGGHTSDYSS SLPSSPNVGH RELRAEAAAV AGLSTPGSLH RAAKRRTSLF ANRRGSDSEK 660
RSLDSRGETT GSGRAIPIKQ SFLLKRSGNS LNKEWKKKYV TLSSNGFLLY HPSINDYIHS 720
THGKEMDLLR TTVKVPGKRP PRAISAFGPS ASINGLVKDM STVQMGEGLE ATTPMPSPSP 780
SPSSLQPPPD QTSKHLLKPD RNLARALSTD CTPSGDLSPL SREPPPSPMV KKQRRKKLTT 840
PSKTEGSAGQ AEAKRKMWKL KSFGSLRNIY KAEENFEFLI VSSTGQTWHF EAASFEERDA 900
WVQAIESQIL ASLQCCESSK VKLRTDSQSE AVAIQAIRNA KGNSICVDCG APNPTWASLN 960
LGALICIECS GIHRNLGTHL SRVRSLDLDD WPRELTLVLT AIGNDTANRV WESDTRGRAK 1020
PSRDSSREER ESWIRAKYEQ LLFLAPLSTS EEPLGRQLWA AVQAQDVATV LLLLAHARHG 1080
PLDTSVEDPQ LRSPLHLAAE LAHVVITQLL LWYGADVAAR DAQGRTALFY ARQAGSQLCA 1140
DILLQHGCPG EGGSAATTPS AATTPSITAT PSPRRRSSAA SVGRADAPVA LV 1192 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0008060; F:ARF GTPase activator activity; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:InterPro.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0030295; F:protein kinase activator activity; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0060749; P:mammary gland alveolus development; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:2001240; P:negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; IEA:Compara.
 GO:0046427; P:positive regulation of JAK-STAT cascade; IEA:Compara.
 GO:0033601; P:positive regulation of mammary gland epithelial cell proliferation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0032312; P:regulation of ARF GTPase activity; IEA:InterPro.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR001164; ArfGAP.
 IPR013684; MIRO-like.
 IPR027417; P-loop_NTPase.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 
Pfam
 PF12796; Ank_2
 PF01412; ArfGap
 PF08477; Miro
 PF00169; PH 
SMART
 SM00248; ANK
 SM00105; ArfGap
 SM00233; PH 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50115; ARFGAP
 PS50003; PH_DOMAIN 
PRINTS
 PR00405; REVINTRACTNG.