CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031484
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Importin-5 
Protein Synonyms/Alias
 cDNA FLJ54573, highly similar to Importin beta-3 
Gene Name
 IPO5 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
27SPDNVVRKQAEETYEubiquitination[1, 2]
228MACGLGGKLVLPMIKubiquitination[1]
311DFAPGFQKKFHEKVIacetylation[2]
311DFAPGFQKKFHEKVIubiquitination[3]
312FAPGFQKKFHEKVIAubiquitination[1]
423IVENAVQKELRLLRGubiquitination[4]
431ELRLLRGKTIECISLubiquitination[1, 2, 5]
488SAWARMCKILGKEFQubiquitination[1]
546DQQSFGIKTAGLEEKubiquitination[6]
553KTAGLEEKSTACQMLubiquitination[1, 2]
565QMLVCYAKELKEGFVubiquitination[1, 2, 4, 7]
568VCYAKELKEGFVEYTubiquitination[1]
675LGGILKAKLEEHFKNubiquitination[1]
681AKLEEHFKNQELRQVubiquitination[1]
838QSADSKTKENVNATEubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 972 AA 
Protein Sequence
MAAAAAEQQQ FYLLLGNLLS PDNVVRKQAE ETYENIPGQS KITFLLQAIR NTTAAEEARQ 60
MAAVLLRRLL SSAFDEVYPA LPSDVQTAIK NLLPGFLQAV NDSCYQNDDS VLKSLVEIAD 120
TVPKYLRPHL EATLQLSLKL CGDTSLNNMQ RQLALEVIVT LSETAAAMLR KHTNIVAQTI 180
PQMLAMMVDL EEDEDWANAD ELEDDDFDSN AVAGESALDR MACGLGGKLV LPMIKEHIMQ 240
MLQNPDWKYR HAGLMALSAI GEGCHQQMEG ILNEIVNFVL LFLQDPHPRV RYAACNAVGQ 300
MATDFAPGFQ KKFHEKVIAA LLQTMEDQGN QRVQAHAAAA LINFTEDCPK SLLIPYLDNL 360
VKHLHSIMVL KLQELIQKGT KLVLEQVVTS IASVADTAEE KFVPYYDLFM PSLKHIVENA 420
VQKELRLLRG KTIECISLIG LAVGKEKFMQ DASDVMQLLL KTQTDFNDME DDDPQISYMI 480
SAWARMCKIL GKEFQQYLPV VMGPLMKTAS IKPEVALLDT QDMENMSDDD GWEFVNLGDQ 540
QSFGIKTAGL EEKSTACQML VCYAKELKEG FVEYTEQVVK LMVPLLKFYF HDGVRVAAAE 600
SMPLLLECAR VRGPEYLTQM WHFMCDALIK AIGTEPDSDV LSEIMHSFAK CIEVMGDGCL 660
NNEHFEELGG ILKAKLEEHF KNQELRQVKR QDEDYDEQVE ESLQDEDDND VYILTKVSDI 720
LHSIFSSYKE KVLPWFEQLL PLIVNLICPH RPWPDRQWGL CIFDDVIEHC SPASFKYAEY 780
FLRPMLQYVC DNSPEVRQAA AYGLGVMAQY GGDNYRPFCT EALPLLVRVI QSADSKTKEN 840
VNATENCISA VGKIMKFKPD CVNVEEVLPH WLSWLPLHED KEEAVQTFNY LCDLIESNHP 900
IVLGPNNTNL PKIFSIIAEG EMHEAIKHED PCAKRLANVV RQVQTSGGLW TECIAQLSPE 960
QQAAIQELLN SA 972 
Gene Ontology
  
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000357; HEAT. 
Pfam
 PF02985; HEAT 
SMART
  
PROSITE
  
PRINTS