CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004850
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 POU domain, class 5, transcription factor 1 
Protein Synonyms/Alias
 NF-A3; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3 
Gene Name
 Pou5f1 
Gene Synonyms/Alias
 Oct-3; Oct-4; Otf-3; Otf3 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
118ADRPNAVKLEKVEPTsumoylation[1, 2]
Reference
 [1] Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1.
 Zhang Z, Liao B, Xu M, Jin Y.
 FASEB J. 2007 Oct;21(12):3042-51. [PMID: 17496161]
 [2] Sumoylation of Oct4 enhances its stability, DNA binding, and transactivation.
 Wei F, Schöler HR, Atchison ML.
 J Biol Chem. 2007 Jul 20;282(29):21551-60. [PMID: 17525163] 
Functional Description
 Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. 
Sequence Annotation
 DOMAIN 131 205 POU-specific.
 DNA_BIND 223 282 Homeobox.
 MOD_RES 106 106 Phosphoserine; by MAPK (By similarity).
 MOD_RES 228 228 Phosphothreonine (By similarity).
 MOD_RES 229 229 Phosphoserine (By similarity).
 MOD_RES 282 282 Phosphoserine (By similarity).
 MOD_RES 347 347 Phosphoserine (By similarity).
 CROSSLNK 118 118 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Developmental protein; DNA-binding; Homeobox; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 352 AA 
Protein Sequence
MAGHLASDFA FSPPPGGGDG SAGLEPGWVD PRTWLSFQGP PGGPGIGPGS EVLGISPCPP 60
AYEFCGGMAY CGPQVGLGLV PQVGVETLQP EGQAGARVES NSEGTSSEPC ADRPNAVKLE 120
KVEPTPEESQ DMKALQKELE QFAKLLKQKR ITLGYTQADV GLTLGVLFGK VFSQTTICRF 180
EALQLSLKNM CKLRPLLEKW VEEADNNENL QEICKSETLV QARKRKRTSI ENRVRWSLET 240
MFLKCPKPSL QQITHIANQL GLEKDVVRVW FCNRRQKGKR SSIEYSQREE YEATGTPFPG 300
GAVSFPLPPG PHFGTPGYGS PHFTTLYSVP FPEGEAFPSV PVTALGSPMH SN 352 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0005667; C:transcription factor complex; IDA:MGI.
 GO:0017053; C:transcriptional repressor complex; IDA:MGI.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
 GO:0000981; F:sequence-specific DNA binding RNA polymerase II transcription factor activity; IDA:BHF-UCL.
 GO:0003714; F:transcription corepressor activity; IDA:MGI.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:UniProtKB.
 GO:0001712; P:ectodermal cell fate commitment; IDA:MGI.
 GO:0001714; P:endodermal cell fate specification; ISO:MGI.
 GO:0030718; P:germ-line stem cell maintenance; IMP:MGI.
 GO:0001710; P:mesodermal cell fate commitment; IDA:MGI.
 GO:0042789; P:mRNA transcription from RNA polymerase II promoter; IDA:BHF-UCL.
 GO:0045955; P:negative regulation of calcium ion-dependent exocytosis; IDA:MGI.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IGI:MGI.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0009786; P:regulation of asymmetric cell division; IEP:BHF-UCL.
 GO:0032526; P:response to retinoic acid; IDA:MGI.
 GO:0001829; P:trophectodermal cell differentiation; IGI:MGI. 
Interpro
 IPR017970; Homeobox_CS.
 IPR001356; Homeodomain.
 IPR009057; Homeodomain-like.
 IPR010982; Lambda_DNA-bd_dom.
 IPR013847; POU.
 IPR015585; POU_dom_5.
 IPR000327; POU_specific. 
Pfam
 PF00046; Homeobox
 PF00157; Pou 
SMART
 SM00389; HOX
 SM00352; POU 
PROSITE
 PS00027; HOMEOBOX_1
 PS50071; HOMEOBOX_2
 PS00035; POU_1
 PS00465; POU_2
 PS51179; POU_3 
PRINTS
 PR00028; POUDOMAIN.