CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037455
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 1,4-alpha-glucan-branching enzyme 
Protein Synonyms/Alias
  
Gene Name
 GBE1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9NLAKVRYKQFSQILKubiquitination[1]
27ENEGGIDKFSRGYESacetylation[2]
27ENEGGIDKFSRGYESubiquitination[1, 3, 4]
102VPHGSKLKVVITSKSubiquitination[1]
108LKVVITSKSGEILYRubiquitination[1, 4, 5, 6]
121YRISPWAKYVVREGDubiquitination[1, 3]
171GISSHEGKVASYKHFubiquitination[1, 3, 4, 5]
176EGKVASYKHFTCNVLubiquitination[1]
401LAMAIPDKWIQLLKEubiquitination[1, 3]
431TNRRYLEKCIAYAESubiquitination[1, 4]
513EWLDFPRKGNNESYHubiquitination[1, 5]
537DDDLLRYKFLNNFDRubiquitination[1, 3, 5]
604VGTALPGKFKIVLDSubiquitination[1]
606TALPGKFKIVLDSDAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 661 AA 
Protein Sequence
MNLAKVRYKQ FSQILKNIGE NEGGIDKFSR GYESFGVHRC ADGGLYCKEW APGAEGVFLT 60
GDFNGWNPFS YPYKKLDYGK WELYIPPKQN KSVLVPHGSK LKVVITSKSG EILYRISPWA 120
KYVVREGDNV NYDWIHWDPE HSYEFKHSRP KKPRSLRIYE SHVGISSHEG KVASYKHFTC 180
NVLPRIKGLG YNCIQLMAIM EHAYYASFGY QITSFFAASS RYGTPEELQE LVDTAHSMGI 240
IVLLDVVHSH ASKNSADGLN MFDGTDSCYF HSGPRGTHDL WDSRLFAYSS WEILRFLLSN 300
IRWWLEEYRF DGFRFDGVTS MLYHHHGVGQ GFSGDYSEYF GLQVDEDALT YLMLANHLVH 360
TLCPDSITIA EDVSGMPALC SPISQGGGGF DYRLAMAIPD KWIQLLKEFK DEDWNMGDIV 420
YTLTNRRYLE KCIAYAESHD QALVGDKSLA FWLMDAEMYT NMSVLTPFTP VIDRGIQLHK 480
MIRLITHGLG GEGYLNFMGN EFGHPEWLDF PRKGNNESYH YARRQFHLTD DDLLRYKFLN 540
NFDRDMNRLE ERYGWLAAPQ AYVSEKHEGN KIIAFERAGL LFIFNFHPSK SYTDYRVGTA 600
LPGKFKIVLD SDAAEYGGHQ RLDHSTDFFS EAFEHNGRPY SLLVYIPSRV ALILQNVDLP 660
N 661 
Gene Ontology
 GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:InterPro.
 GO:0043169; F:cation binding; IEA:InterPro.
 GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
 GO:0005978; P:glycogen biosynthetic process; IEA:InterPro. 
Interpro
 IPR006407; 1-4-A-glucan_branch_enz.
 IPR006048; A-amylase_b_C.
 IPR015902; Glyco_hydro_13.
 IPR013780; Glyco_hydro_13_b.
 IPR006047; Glyco_hydro_13_cat_dom.
 IPR004193; Glyco_hydro_13_N.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR017853; Glycoside_hydrolase_SF.
 IPR013783; Ig-like_fold.
 IPR014756; Ig_E-set. 
Pfam
 PF00128; Alpha-amylase
 PF02806; Alpha-amylase_C
 PF02922; CBM_48 
SMART
  
PROSITE
  
PRINTS