CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000056
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cell division cycle 7-related protein kinase 
Protein Synonyms/Alias
 CDC7-related kinase; HsCdc7; huCdc7 
Gene Name
 CDC7 
Gene Synonyms/Alias
 CDC7L1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
29FQAEGSLKKNEQNFKubiquitination[1, 2]
30QAEGSLKKNEQNFKLubiquitination[2]
90PEEKIALKHLIPTSHubiquitination[2]
179GIVHRDVKPSNFLYNubiquitination[2]
191LYNRRLKKYALVDFGubiquitination[2]
207AQGTHDTKIELLKFVubiquitination[2]
212DTKIELLKFVQSEAQubiquitination[1, 2]
227QERCSQNKSHIITGNubiquitination[2]
235SHIITGNKIPLSGPVubiquitination[2]
253LDQQSTTKASVKRPYubiquitination[2]
274IKQGKDGKEGSVGLSubiquitination[2]
312VKLMKQSKTVDVLSRubiquitination[2]
331KKKAISTKVMNSAVMubiquitination[2]
340MNSAVMRKTASSCPAubiquitination[2]
358CDCYATDKVCSICLSubiquitination[2]
441RETIQAAKTFGKSILubiquitination[2]
445QAAKTFGKSILCSKEubiquitination[2]
451GKSILCSKEVPAQDLubiquitination[2]
492HQPAISEKTDHKASCubiquitination[2]
570ALLHPFFKDMSL***ubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3. 
Sequence Annotation
 DOMAIN 58 574 Protein kinase.
 NP_BIND 64 72 ATP (By similarity).
 ACT_SITE 177 177 Proton acceptor (By similarity).
 BINDING 90 90 ATP (By similarity).
 MOD_RES 27 27 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Cell cycle; Cell division; Complete proteome; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 574 AA 
Protein Sequence
MEASLGIQMD EPMAFSPQRD RFQAEGSLKK NEQNFKLAGV KKDIEKLYEA VPQLSNVFKI 60
EDKIGEGTFS SVYLATAQLQ VGPEEKIALK HLIPTSHPIR IAAELQCLTV AGGQDNVMGV 120
KYCFRKNDHV VIAMPYLEHE SFLDILNSLS FQEVREYMLN LFKALKRIHQ FGIVHRDVKP 180
SNFLYNRRLK KYALVDFGLA QGTHDTKIEL LKFVQSEAQQ ERCSQNKSHI ITGNKIPLSG 240
PVPKELDQQS TTKASVKRPY TNAQIQIKQG KDGKEGSVGL SVQRSVFGER NFNIHSSISH 300
ESPAVKLMKQ SKTVDVLSRK LATKKKAIST KVMNSAVMRK TASSCPASLT CDCYATDKVC 360
SICLSRRQQV APRAGTPGFR APEVLTKCPN QTTAIDMWSA GVIFLSLLSG RYPFYKASDD 420
LTALAQIMTI RGSRETIQAA KTFGKSILCS KEVPAQDLRK LCERLRGMDS STPKLTSDIQ 480
GHASHQPAIS EKTDHKASCL VQTPPGQYSG NSFKKGDSNS CEHCFDEYNT NLEGWNEVPD 540
EAYDLLDKLL DLNPASRITA EEALLHPFFK DMSL 574 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HGNC.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016301; F:kinase activity; IDA:HGNC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0006260; P:DNA replication; TAS:Reactome.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome.
 GO:0008284; P:positive regulation of cell proliferation; IMP:HGNC.
 GO:0033261; P:regulation of S phase; IMP:HGNC. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS