UniProt Accession

 FBX32_HUMAN; Q969P5; A4KYM0 

Genbank Protein ID

 AY059629; AJ420108; EF143260; AK056986; AC090193 

Genbank Nucleotide ID

 AAL16407.1; CAD12251.1; ABO37797.1; BAB71333.1 

Protein Name

 F-box only protein 32 

Protein Synonyms/Alias

 Atrogin-1; Muscle atrophy F-box protein; MAFbx 

Gene Name

 FBXO32 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
17	SPGQNWVKTADGWKR	ubiquitination	[1]
29	WKRFLDEKSGSFVSD	ubiquitination	[2]
48	CNKEVYNKENLFNSL	ubiquitination	[1, 2, 3]
143	SLSGIAQKNFMNILE	ubiquitination	[2]
268	EDRLLWKKLCQYHFS	ubiquitination	[3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. 

Sequence Annotation

 DOMAIN 223 271 F-box.
 MOTIF 62 67 Nuclear localization signal.
 MOTIF 169 173 Nuclear export signal.
 MOTIF 280 295 Bipartite nuclear localization signal.  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; Nucleus; Polymorphism; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 355 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway. 

Interpro

 IPR001810; F-box_dom_cyclin-like. 

Pfam

  

SMART

  

PROSITE

 PS50181; FBOX 

PRINTS