CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022191
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Integrator complex subunit 9 
Protein Synonyms/Alias
 Int9; Protein related to CPSF subunits of 74 kDa; RC-74 
Gene Name
 INTS9 
Gene Synonyms/Alias
 RC74 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
2******MKLYCLSGHubiquitination[1]
18TLPCNVLKFKSTTIMubiquitination[1]
58NLPGWSLKDGNAFLDubiquitination[1, 2, 3, 4]
66DGNAFLDKELKECSGubiquitination[1]
149NFIERVPKAQSASLWubiquitination[1]
157AQSASLWKNKDIQRLubiquitination[1]
159SASLWKNKDIQRLLPubiquitination[1]
374LIQTNKLKHYPSIHGubiquitination[1]
459LNFIQVSKLLKEVQPubiquitination[1, 2, 4, 5]
462IQVSKLLKEVQPLHVubiquitination[1]
510EVLALPFKRRYEKIEubiquitination[1, 2, 4]
569PTSGKKRKRVSDDVPubiquitination[1]
579SDDVPDCKVLKPLLSubiquitination[1]
582VPDCKVLKPLLSGSIubiquitination[1]
607KHGFSDIKVEDTAKGubiquitination[1]
653RLRDLVLKFLQKF**ubiquitination[1, 2, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. 
Sequence Annotation
  
Keyword
 Alternative splicing; Complete proteome; Nucleus; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 658 AA 
Protein Sequence
MKLYCLSGHP TLPCNVLKFK STTIMLDCGL DMTSTLNFLP LPLVQSPRLS NLPGWSLKDG 60
NAFLDKELKE CSGHVFVDSV PEFCLPETEL IDLSTVDVIL ISNYHCMMAL PYITEHTGFT 120
GTVYATEPTV QIGRLLMEEL VNFIERVPKA QSASLWKNKD IQRLLPSPLK DAVEVSTWRR 180
CYTMQEVNSA LSKIQLVGYS QKIELFGAVQ VTPLSSGYAL GSSNWIIQSH YEKVSYVSGS 240
SLLTTHPQPM DQASLKNSDV LVLTGLTQIP TANPDGMVGE FCSNLALTVR NGGNVLVPCY 300
PSGVIYDLLE CLYQYIDSAG LSSVPLYFIS PVANSSLEFS QIFAEWLCHN KQSKVYLPEP 360
PFPHAELIQT NKLKHYPSIH GDFSNDFRQP CVVFTGHPSL RFGDVVHFME LWGKSSLNTV 420
IFTEPDFSYL EALAPYQPLA MKCIYCPIDT RLNFIQVSKL LKEVQPLHVV CPEQYTQPPP 480
AQSHRMDLMI DCQPPAMSYR RAEVLALPFK RRYEKIEIMP ELADSLVPME IKPGISLATV 540
SAVLHTKDNK HLLQPPPRPA QPTSGKKRKR VSDDVPDCKV LKPLLSGSIP VEQFVQTLEK 600
HGFSDIKVED TAKGHIVLLQ EAETLIQIEE DSTHIICDND EMLRVRLRDL VLKFLQKF 658 
Gene Ontology
 GO:0032039; C:integrator complex; IDA:HGNC.
 GO:0016787; F:hydrolase activity; IEA:InterPro.
 GO:0016180; P:snRNA processing; IDA:HGNC. 
Interpro
 IPR001279; Beta-lactamas-like.
 IPR022712; Beta_Casp.
 IPR027074; Integrator_9su. 
Pfam
 PF10996; Beta-Casp 
SMART
 SM01027; Beta-Casp 
PROSITE
  
PRINTS