CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013504
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 PAB-dependent poly(A)-specific ribonuclease subunit 3 
Protein Synonyms/Alias
 hPan3 
Gene Name
 PAN3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
116GPPPGPKKPDLGDPGubiquitination[1]
249RLVPMGSKARKAKNPubiquitination[1]
296TASEFIPKGGSTSRLubiquitination[1, 2]
513CYKAVNSKDDLPYCLubiquitination[1]
594NADAYFTKRKWGQHEubiquitination[1, 2, 3, 4, 5]
645CRVMDPTKILITGKTubiquitination[2, 4]
773VIEEDLAKEVQNGRLubiquitination[1, 2]
798NERPEFQKDPTWSETubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Functions in cytoplasmic mRNA decay. As part of the Pan nuclease complex, recruits polyadenylate-binding protein which in turn stimulates PAN2 nuclease activity. 
Sequence Annotation
 DOMAIN 492 754 Protein kinase.
 ZN_FING 43 71 C3H1-type.
 REGION 1 491 Interaction with polyadenylate-binding
 REGION 492 887 Interaction with PAN2.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Metal-binding; Nonsense-mediated mRNA decay; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 887 AA 
Protein Sequence
MNSGGGLPPP SAAASPSSSS LAAAVAVVAP PGVGGVPGGA AVGVKLKYCR YYAKDKTCFY 60
GEECQFLHED PAAGAAPGLG LHSNSVPLAL AGAPVAGFPP GAVAGGGAGP PPGPKKPDLG 120
DPGTGAAAGG GGSSGGLDGP RLAIPGMDGG ALTDTSLTDS YFSTSFIGVN GFGSPVETKY 180
PLMQRMTNSS SSPSLLNDSA KPYSAHDPLT SPASSLFNDF GALNISQRRK PRKYRLGMLE 240
ERLVPMGSKA RKAKNPIGCL ADRCKSGVPI NMVWWNRVTE NNLQTPNPTA SEFIPKGGST 300
SRLSNVSQSN MSAFSQVFSH PSMGSPATAG LAPGMSLSAG SSPLHSPKIT PHTSPAPRRR 360
SHTPNPASYM VPSSASTSVN NPVSQTPSSG QVIQKETVGG TTYFYTDTTP APLTGMVFPN 420
YHIYPPTAPH VAYMQPKANA PSFFMADELR QELINRHLIT MAQIDQADMP AVPTEVDSYH 480
SLFPLEPLPP PNRIQKSSNF GYITSCYKAV NSKDDLPYCL RRIHGFRLVN TKCMVLVDMW 540
KKIQHSNIVT LREVFTTKAF AEPSLVFAYD FHAGGETMMS RHFNDPNADA YFTKRKWGQH 600
EGPLPRQHAG LLPESLIWAY IVQLSSALRT IHTAGLACRV MDPTKILITG KTRLRVNCVG 660
VFDVLTFDNS QNNNPLALMA QYQQADLISL GKVVLALACN SLAGIQRENL QKAMELVTIN 720
YSSDLKNLIL YLLTDQNRMR SVNDIMPMIG ARFYTQLDAA QMRNDVIEED LAKEVQNGRL 780
FRLLAKLGTI NERPEFQKDP TWSETGDRYL LKLFRDHLFH QVTEAGAPWI DLSHIISCLN 840
KLDAGVPEKI SLISRDEKSV LVVTYSDLKR CFENTFQELI AAANGQL 887 
Gene Ontology
 GO:0005813; C:centrosome; IDA:HPA.
 GO:0000932; C:cytoplasmic mRNA processing body; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031251; C:PAN complex; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0004672; F:protein kinase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0000290; P:deadenylation-dependent decapping of nuclear-transcribed mRNA; IEA:Compara.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
 GO:0010606; P:positive regulation of cytoplasmic mRNA processing body assembly; IEA:Compara.
 GO:0006605; P:protein targeting; IEA:Compara. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR000571; Znf_CCCH. 
Pfam
  
SMART
 SM00356; ZnF_C3H1 
PROSITE
 PS50011; PROTEIN_KINASE_DOM
 PS50103; ZF_C3H1 
PRINTS