UniProt Accession

 COIL_HUMAN; P38432; B2R931 

Genbank Protein ID

 M58411; AK313616; AC004584; CH471109; BC010385; U06632 

Genbank Nucleotide ID

 AAA36412.1; BAG36378.1; EAW94522.1; AAH10385.1; AAB81550.1 

Protein Name

 Coilin 

Protein Synonyms/Alias

 p80 

Gene Name

 COIL 

Gene Synonyms/Alias

 CLN80 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
293	TKNTTADKLAIKLGF	acetylation	[1]
405	EENLFSWKGAKGRGM	acetylation	[2]
476	AAPQVGEKIAFKLLE	ubiquitination	[3]
496	SPDVSDYKEGRILSH	ubiquitination	[3, 4, 5]
555	SKITVFWKELIDPRL	ubiquitination	[6, 7]

Reference

 [1] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Is a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin. 

Sequence Annotation

 REPEAT 223 226 1-1.
 REPEAT 268 271 1-2.
 REPEAT 386 389 2-1.
 REPEAT 413 414 3-1.
 REPEAT 415 416 3-2.
 REPEAT 417 418 3-3.
 REPEAT 419 420 3-4.
 DOMAIN 460 559 Tudor; atypical.
 REPEAT 517 520 2-2.
 REGION 223 271 2 X 4 AA repeats of A-R-N-S.
 REGION 386 520 2 X 4 AA repeats of S-L-P-A.
 REGION 413 420 4 X 2 AA tandem repeats of R-G.
 MOD_RES 105 105 Phosphoserine.
 MOD_RES 122 122 Phosphothreonine.
 MOD_RES 184 184 Phosphoserine; by VRK1 and VRK2.
 MOD_RES 271 271 Phosphoserine.
 MOD_RES 272 272 Phosphoserine.
 MOD_RES 290 290 Phosphothreonine.
 MOD_RES 301 301 Phosphoserine.
 MOD_RES 303 303 Phosphothreonine.
 MOD_RES 456 456 Phosphothreonine.
 MOD_RES 489 489 Phosphoserine.
 MOD_RES 566 566 Phosphoserine.  

Keyword

 Complete proteome; Methylation; Nucleus; Phosphoprotein; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 576 AA 

Protein Sequence

Gene Ontology

 GO:0015030; C:Cajal body; IDA:UniProtKB.
 GO:0001674; C:female germ cell nucleus; IEA:Compara.
 GO:0005730; C:nucleolus; IDA:UniProtKB.
 GO:0015036; F:disulfide oxidoreductase activity; IEA:Compara. 

Interpro

 IPR024822; Coilin. 

Pfam

  

SMART

  

PROSITE

 PS50304; TUDOR 

PRINTS