CPLM-002363

Genbank Nucleotide ID

Eukaryotic translation initiation factor 4E

Protein Synonyms/Alias

eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

Homo sapiens (Human)

Position	Peptide	Type	References
36	ANPEHYIKHPLQNRW	sumoylation	[1, 2]
36	ANPEHYIKHPLQNRW	ubiquitination	[3]
49	RWALWFFKNDKSKTW	sumoylation	[1, 2]
119	RWLITLNKQQRRSDL	ubiquitination	[4]
162	NVRAKGDKIAIWTTE	sumoylation	[1, 2]
206	SHADTATKSGSTTKN	sumoylation	[1, 2]
212	TKSGSTTKNRFVV**	sumoylation	[1, 2]

[1] Sumoylation of eIF4E activates mRNA translation.
Xu X, Vatsyayan J, Gao C, Bakkenist CJ, Hu J.
EMBO Rep. 2010 Apr;11(4):299-304. [PMID: 20224576]
[2] HDAC2 promotes eIF4E sumoylation and activates mRNA translation gene specifically.
Xu X, Vatsyayan J, Gao C, Bakkenist CJ, Hu J.
J Biol Chem. 2010 Jun 11;285(24):18139-43. [PMID: 20421305]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]

Functional Description

Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

REGION 37 40 EIF4EBP1/2/3 binding.
REGION 56 57 7-methylguanosine-containing mRNA cap
REGION 73 77 EIF4EBP1/2/3 binding.
REGION 102 103 7-methylguanosine-containing mRNA cap
REGION 132 139 EIF4EBP1/2/3 binding.
REGION 157 162 7-methylguanosine-containing mRNA cap
REGION 205 207 7-methylguanosine-containing mRNA cap
MOD_RES 2 2 N-acetylalanine.
MOD_RES 209 209 Phosphoserine; by PKC and MKNK2.

3D-structure; Acetylation; Alternative splicing; Chromosomal rearrangement; Complete proteome; Cytoplasm; Direct protein sequencing; Host-virus interaction; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding; Translation regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000932; C:cytoplasmic mRNA processing body; IDA:MGI.
GO:0010494; C:cytoplasmic stress granule; IDA:UniProtKB.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0016281; C:eukaryotic translation initiation factor 4F complex; TAS:ProtInc.
GO:0005845; C:mRNA cap binding complex; IDA:UniProtKB.
GO:0016442; C:RNA-induced silencing complex; IDA:MGI.
GO:0000339; F:RNA cap binding; TAS:ProtInc.
GO:0003743; F:translation initiation factor activity; ISS:UniProtKB.
GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
GO:0000082; P:G1/S transition of mitotic cell cycle; IMP:UniProtKB.
GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
GO:0030324; P:lung development; IEA:Compara.
GO:0006406; P:mRNA export from nucleus; TAS:Reactome.
GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
GO:0045931; P:positive regulation of mitotic cell cycle; IMP:UniProtKB.
GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW.

IPR023398; TIF_eIF4e-like_dom.
IPR001040; TIF_eIF_4E.
IPR019770; TIF_eIF_4E_CS.