UniProt Accession

 PUF60_HUMAN; Q9UHX1; A8K8K8; Q969E7; Q96D94; Q96H63; Q99628; Q9NZA0; Q9UJY7 

Genbank Protein ID

 AF217197; AF114818; AK292373; CR457143; CH471162; CH471162; BC008875; BC009734; BC011265; BC011979; U51586; AF190744 

Genbank Nucleotide ID

 AAF27522.2; AAF23589.1; BAF85062.1; CAG33424.1; EAW82187.1; EAW82189.1; AAH08875.1; AAH09734.1; AAH11265.1; AAH11979.1; AAB41656.1; AAF05605.1 

Protein Name

 Poly(U)-binding-splicing factor PUF60 

Protein Synonyms/Alias

 60 kDa poly(U)-binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-binding protein 1; RoBP1; Siah-binding protein 1; Siah-BP1 

Gene Name

 PUF60 

Gene Synonyms/Alias

 FIR; ROBPI; SIAHBP1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
172	MLGGRNIKVGRPSNI	ubiquitination	[1]
214	DLSDDDIKSVFEAFG	ubiquitination	[1]
224	FEAFGKIKSCTLARD	ubiquitination	[1]
238	DPTTGKHKGYGFIEY	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA. 

Sequence Annotation

 DOMAIN 129 207 RRM 1.
 DOMAIN 226 304 RRM 2.
 DOMAIN 462 549 RRM 3; atypical.
 REGION 1 516 Inhibits homodimerization.
 REGION 77 559 Inhibits transcriptional repression,
 MOD_RES 60 60 Phosphothreonine.
 MOD_RES 112 112 Phosphoserine.
 MOD_RES 251 251 N6-acetyllysine.
 MOD_RES 314 314 Phosphothreonine.
 MOD_RES 454 454 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Apoptosis; Complete proteome; Direct protein sequencing; DNA-binding; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Ribonucleoprotein; RNA-binding; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 559 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:HPA.
 GO:0030529; C:ribonucleoprotein complex; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR006532; PolyU-bd.
 IPR000504; RRM_dom.
 IPR003954; RRM_dom_euk. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM
 SM00361; RRM_1 

PROSITE

 PS50102; RRM 

PRINTS