CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008181
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CCAAT/enhancer-binding protein alpha 
Protein Synonyms/Alias
 C/EBP alpha 
Gene Name
 CEBPA 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
161ALRPLVIKQEPREEDacetylation[1]
161ALRPLVIKQEPREEDsumoylation[2, 3]
161ALRPLVIKQEPREEDubiquitination[4, 5]
313RNVETQQKVLELTSDubiquitination[4, 5]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy through its PIASy-enhanced modification by SUMO-1 or SUMO-3.
 Subramanian L, Benson MD, Iñiguez-Lluhí JA.
 J Biol Chem. 2003 Mar 14;278(11):9134-41. [PMID: 12511558]
 [3] Target proteins of C/EBPalphap30 in AML: C/EBPalphap30 enhances sumoylation of C/EBPalphap42 via up-regulation of Ubc9.
 Geletu M, Balkhi MY, Peer Zada AA, Christopeit M, Pulikkan JA, Trivedi AK, Tenen DG, Behre G.
 Blood. 2007 Nov 1;110(9):3301-9. [PMID: 17671234]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. 
Sequence Annotation
 DOMAIN 282 345 bZIP.
 REGION 286 313 Basic motif (By similarity).
 REGION 317 345 Leucine-zipper (By similarity).
 MOD_RES 161 161 N6-acetyllysine.  
Keyword
 Acetylation; Activator; Complete proteome; DNA-binding; Host-virus interaction; Nucleus; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 358 AA 
Protein Sequence
MESADFYEAE PRPPMSSHLQ SPPHAPSSAA FGFPRGAGPA QPPAPPAAPE PLGGICEHET 60
SIDISAYIDP AAFNDEFLAD LFQHSRQQEK AKAAVGPTGG GGGGDFDYPG APAGPGGAVM 120
PGGAHGPPPG YGCAAAGYLD GRLEPLYERV GAPALRPLVI KQEPREEDEA KQLALAGLFP 180
YQPPPPPPPS HPHPHPPPAH LAAPHLQFQI AHCGQTTMHL QPGHPTPPPT PVPSPHPAPA 240
LGAAGLPGPG SALKGLGAAH PDLRASGGSG AGKAKKSVDK NSNEYRVRRE RNNIAVRKSR 300
DKAKQRNVET QQKVLELTSD NDRLRKRVEQ LSRELDTLRG IFRQLPESSL VKAMGNCA 358 
Gene Ontology
 GO:0016363; C:nuclear matrix; IEA:Compara.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0035189; C:Rb-E2F complex; IEA:Compara.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; NAS:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:UniProtKB.
 GO:0006953; P:acute-phase response; IEA:Compara.
 GO:0050873; P:brown fat cell differentiation; IEA:Compara.
 GO:0048469; P:cell maturation; IEA:Compara.
 GO:0071285; P:cellular response to lithium ion; IEA:Compara.
 GO:0071407; P:cellular response to organic cyclic compound; IEA:Compara.
 GO:0008203; P:cholesterol metabolic process; IEA:Compara.
 GO:0019221; P:cytokine-mediated signaling pathway; NAS:UniProtKB.
 GO:0001892; P:embryonic placenta development; IEA:Compara.
 GO:0006091; P:generation of precursor metabolites and energy; TAS:ProtInc.
 GO:0048839; P:inner ear development; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0030324; P:lung development; IEA:Compara.
 GO:0030225; P:macrophage differentiation; IEA:Compara.
 GO:0007005; P:mitochondrion organization; IEA:Compara.
 GO:0030099; P:myeloid cell differentiation; NAS:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0031100; P:organ regeneration; IEA:Compara.
 GO:0045600; P:positive regulation of fat cell differentiation; IEA:Compara.
 GO:0045669; P:positive regulation of osteoblast differentiation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045945; P:positive regulation of transcription from RNA polymerase III promoter; IDA:UniProtKB.
 GO:0051384; P:response to glucocorticoid stimulus; IEA:Compara.
 GO:0033274; P:response to vitamin B2; IEA:Compara.
 GO:0000050; P:urea cycle; IEA:Compara.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW.
 GO:0050872; P:white fat cell differentiation; IEA:Compara. 
Interpro
 IPR004827; bZIP.
 IPR016468; CCAAT/enhancer-binding. 
Pfam
 PF07716; bZIP_2 
SMART
 SM00338; BRLZ 
PROSITE
 PS50217; BZIP
 PS00036; BZIP_BASIC 
PRINTS