UniProt Accession

 GLSL_MOUSE; Q571F8; B2RUF2; Q3TMJ6 

Genbank Protein ID

 AK220231; AK165896; BC141115 

Genbank Nucleotide ID

 BAD90156.1; BAE38445.1; AAI41116.1 

Protein Name

 Glutaminase liver isoform, mitochondrial 

Protein Synonyms/Alias

 GLS; L-glutaminase; L-glutamine amidohydrolase 

Gene Name

 Gls2 

Gene Synonyms/Alias

 Kiaa4146 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
91	QERIPIHKFTTALKA	ubiquitination	[1]
97	HKFTTALKATGLQTS	ubiquitination	[1]
151	LTQAFRKKFVIPDFE	ubiquitination	[1]
172	DRIFEDAKEPTGGKV	acetylation	[2]
172	DRIFEDAKEPTGGKV	ubiquitination	[1]
188	AYIPHLAKSNPDLWG	acetylation	[3]
244	YVHKFVGKEPSGLRY	acetylation	[2]
244	YVHKFVGKEPSGLRY	ubiquitination	[1]
253	PSGLRYNKLSLNEEG	acetylation	[4]
253	PSGLRYNKLSLNEEG	succinylation	[4]
253	PSGLRYNKLSLNEEG	ubiquitination	[1]
279	IVVSSLIKMDCNKAE	acetylation	[3, 5]
284	LIKMDCNKAEKFDFV	acetylation	[3, 5]
329	YAIGYYLKEKKCFPK	acetylation	[2, 3, 5]
329	YAIGYYLKEKKCFPK	ubiquitination	[1]
515	SAMDMEQKDYDSRTA	ubiquitination	[1]
543	KFLIEACKVNPFVKD	ubiquitination	[1]
549	CKVNPFVKDRWGNIP	acetylation	[2]
549	CKVNPFVKDRWGNIP	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

 Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production (By similarity). 

Sequence Annotation

 REPEAT 518 551 ANK 1.
 REPEAT 552 585 ANK 2.
 BINDING 219 219 Substrate (By similarity).
 BINDING 268 268 Substrate (By similarity).
 BINDING 314 314 Substrate (By similarity).
 BINDING 321 321 Substrate (By similarity).
 BINDING 347 347 Substrate (By similarity).
 BINDING 399 399 Substrate (By similarity).
 BINDING 417 417 Substrate; via amide nitrogen (By  

Keyword

 ANK repeat; Complete proteome; Hydrolase; Mitochondrion; Reference proteome; Repeat; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 602 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0004359; F:glutaminase activity; IEA:EC.
 GO:0006541; P:glutamine metabolic process; IEA:InterPro.
 GO:0072593; P:reactive oxygen species metabolic process; IEA:Compara.
 GO:0042981; P:regulation of apoptotic process; IEA:Compara. 

Interpro

 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR012338; Beta-lactam/transpept-like.
 IPR015868; Glutaminase. 

Pfam

 PF12796; Ank_2
 PF04960; Glutaminase 

SMART

 SM00248; ANK 

PROSITE

 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 

PRINTS