CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006570
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NAD-dependent malic enzyme, mitochondrial 
Protein Synonyms/Alias
 NAD-ME 
Gene Name
 MAE1 
Gene Synonyms/Alias
 YKL029C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
58TRENTFQKPYSDEEVacetylation[1]
67YSDEEVTKTPVGSRAacetylation[1]
151YKQLCYLKTPLAKNDacetylation[1]
156YLKTPLAKNDFMTSLacetylation[1]
313QYDDFLEKFIKAVKKacetylation[1]
335HFEDFGVKNARRLLEacetylation[1]
631GAPGETVKVPRDFDEacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
  
Sequence Annotation
 ACT_SITE 187 187 Proton donor (By similarity).
 ACT_SITE 259 259 Proton acceptor (By similarity).
 METAL 330 330 Divalent metal cation (By similarity).
 METAL 331 331 Divalent metal cation (By similarity).
 METAL 354 354 Divalent metal cation (By similarity).
 BINDING 354 354 NAD (By similarity).
 BINDING 499 499 NAD (By similarity).  
Keyword
 Complete proteome; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 669 AA 
Protein Sequence
MLRTRLSVSV AARSQLTRSL TASRTAPLRR WPIQQSRLYS SNTRSHKATT TRENTFQKPY 60
SDEEVTKTPV GSRARKIFEA PHPHATRLTV EGAIECPLES FQLLNSPLFN KGSAFTQEER 120
EAFNLEALLP PQVNTLDEQL ERSYKQLCYL KTPLAKNDFM TSLRVQNKVL YFALIRRHIK 180
ELVPIIYTPT EGDAIAAYSH RFRKPEGVFL DITEPDSIEC RLATYGGDKD VDYIVVSDSE 240
GILGIGDQGI GGVRIAISKL ALMTLCGGIH PGRVLPVCLD VGTNNKKLAR DELYMGNKFS 300
RIRGKQYDDF LEKFIKAVKK VYPSAVLHFE DFGVKNARRL LEKYRYELPS FNDDIQGTGA 360
VVMASLIAAL KHTNRDLKDT RVLIYGAGSA GLGIADQIVN HMVTHGVDKE EARKKIFLMD 420
RRGLILQSYE ANSTPAQHVY AKSDAEWAGI NTRSLHDVVE NVKPTCLVGC STQAGAFTQD 480
VVEEMHKHNP RPIIFPLSNP TRLHEAVPAD LMKWTNNNAL VATGSPFPPV DGYRISENNN 540
CYSFPGIGLG AVLSRATTIT DKMISAAVDQ LAELSPLREG DSRPGLLPGL DTITNTSARL 600
ATAVILQALE EGTARIEQEQ VPGGAPGETV KVPRDFDECL QWVKAQMWEP VYRPMIKVQH 660
DPSVHTNQL 669 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0016619; F:malate dehydrogenase (oxaloacetate-decarboxylating) activity; IEA:EC.
 GO:0004470; F:malic enzyme activity; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0006520; P:cellular amino acid metabolic process; IMP:SGD.
 GO:0006108; P:malate metabolic process; IEA:InterPro.
 GO:0006090; P:pyruvate metabolic process; IMP:SGD. 
Interpro
 IPR015884; Malic_enzyme_CS.
 IPR012301; Malic_N.
 IPR012302; Malic_NAD-bd.
 IPR001891; Malic_OxRdtase.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00390; malic
 PF03949; Malic_M 
SMART
 SM00919; Malic_M 
PROSITE
 PS00331; MALIC_ENZYMES 
PRINTS
 PR00072; MALOXRDTASE.