CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011470
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein PUN1 
Protein Synonyms/Alias
 Plasma membrane protein up-regulated during nitrogen stress 1 
Gene Name
 PUN1 
Gene Synonyms/Alias
 YLR414C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
260ANAPIEEKPLI****ubiquitination[1, 2, 3, 4, 5]
Reference
 [1] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [2] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [4] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [5] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Contributes to the wild-type cellular response to nitrogen stress through signaling pathways that regulate the expression of genes involved in amino acid biosynthesis. Required for wild-type filamentous growth, cell growth, and cell-cell adhesion. 
Sequence Annotation
 CARBOHYD 100 100 N-linked (GlcNAc...) (Potential).
 CARBOHYD 209 209 N-linked (GlcNAc...) (Potential).
 CROSSLNK 260 260 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Cell membrane; Complete proteome; Glycoprotein; Isopeptide bond; Membrane; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 263 AA 
Protein Sequence
MRNFFTLFFA AIFSLGALIL AIVACAGSTK NYSPINKIYC AELDLSQMKV STVLPSLSSA 60
TLSSLGLPSY INIGLWSYCT VDSSHNIQSC SSPHGIQNFN LSSLVYDNIN NNEALELMDS 120
VASVVLPEKL KSKMTYYNNL VKCMFITILI GIVLTFVNLV FNVLRWIIHI RPLTWFGAFF 180
SFFAFAALLV SIGSCLGTYS YIKYILKHNY SDYGISMSIG RNYQGLMWGA VVGALLNFIL 240
WCSVRSRPTV IYANAPIEEK PLI 263 
Gene Ontology
 GO:0005933; C:cellular bud; IDA:SGD.
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005923; C:tight junction; IEA:InterPro.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0031505; P:fungal-type cell wall organization; IMP:SGD.
 GO:0051259; P:protein oligomerization; IDA:SGD. 
Interpro
 IPR017974; Claudin_CS.
 IPR009571; SUR7/PalI. 
Pfam
 PF06687; SUR7 
SMART
  
PROSITE
  
PRINTS