UniProt Accession

 DDAH1_RAT; O08557 

Genbank Protein ID

 D86041 

Genbank Nucleotide ID

 BAA18993.1 

Protein Name

 N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 

Protein Synonyms/Alias

 DDAH-1; Dimethylarginine dimethylaminohydrolase 1; DDAHI; Dimethylargininase-1 

Gene Name

 Ddah1 

Gene Synonyms/Alias

 Ddah 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
100	PGAPSRRKEVDMMKE	acetylation	[1]
106	RKEVDMMKEALEKLQ	acetylation	[1]
144	EFFVGLSKRTNQRGA	acetylation	[1]
199	ESAQKALKIMQQMSD	acetylation	[1]
247	EEYPESAKVYEKLKD	acetylation	[1]
251	ESAKVYEKLKDHLLI	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. 

Sequence Annotation

 ACT_SITE 173 173 Proton donor (By similarity).
 ACT_SITE 274 274 Nucleophile (By similarity).
 METAL 274 274 Zinc (By similarity).
 BINDING 30 30 Substrate; via carbonyl oxygen (By
 BINDING 73 73 Substrate (By similarity).
 BINDING 78 78 Substrate (By similarity).
 BINDING 79 79 Substrate (By similarity).
 BINDING 98 98 Substrate (By similarity).
 BINDING 145 145 Substrate (By similarity).
 BINDING 268 268 Substrate; via carbonyl oxygen (By
 MOD_RES 2 2 N-acetylalanine.  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Hydrolase; Metal-binding; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 285 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0016597; F:amino acid binding; IDA:RGD.
 GO:0016403; F:dimethylargininase activity; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006525; P:arginine metabolic process; IDA:RGD.
 GO:0000052; P:citrulline metabolic process; ISS:UniProtKB.
 GO:0045766; P:positive regulation of angiogenesis; IMP:RGD.
 GO:0045429; P:positive regulation of nitric oxide biosynthetic process; IMP:RGD.
 GO:0003073; P:regulation of systemic arterial blood pressure; IEA:Compara. 

Interpro

 IPR003198; Amidino_trans. 

Pfam

 PF02274; Amidinotransf 

SMART

  

PROSITE

  

PRINTS