UniProt Accession

 HMCS2_RAT; P22791 

Genbank Protein ID

 M33648; M63800 

Genbank Nucleotide ID

 AAA41336.1 

Protein Name

 Hydroxymethylglutaryl-CoA synthase, mitochondrial 

Protein Synonyms/Alias

 HMG-CoA synthase; 3-hydroxy-3-methylglutaryl coenzyme A synthase 

Gene Name

 Hmgcs2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
83	FNNVEAGKYTVGLGQ	acetylation	[1]
118	QRLMERTKLPWDAVG	acetylation	[1]
243	ENAYDFYKPNLASEY	acetylation	[1]
256	EYPLVDGKLSIQCYL	acetylation	[1]
310	PFCKMVQKSLARLMF	acetylation	[1]
327	FLSSSSDKQNNLYKG	acetylation	[1]
333	DKQNNLYKGLEAFKG	acetylation	[1]
339	YKGLEAFKGLKLEET	acetylation	[1]
342	LEAFKGLKLEETYTN	acetylation	[1]
350	LEETYTNKDVDKALL	acetylation	[1]
354	YTNKDVDKALLKASL	acetylation	[1]
358	DVDKALLKASLDMFN	acetylation	[1]
366	ASLDMFNKKTKASLY	acetylation	[1]
427	FFSFRVSKDASPGSP	acetylation	[1]
437	SPGSPLEKLVSSVSD	acetylation	[1]
447	SSVSDLPKRLDSRRR	acetylation	[1]
473	QREQFYHKVNFSPPG	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. 

Sequence Annotation

 ACT_SITE 132 132 Proton donor/acceptor (By similarity).
 ACT_SITE 166 166 Nucleophile (By similarity).
 ACT_SITE 301 301 Proton donor/acceptor (By similarity).
 BINDING 80 80 Substrate (By similarity).
 BINDING 204 204 Substrate (By similarity).
 BINDING 258 258 Substrate (By similarity).
 BINDING 380 380 Substrate (By similarity).
 MOD_RES 83 83 N6-acetyllysine (By similarity).
 MOD_RES 310 310 N6-succinyllysine (By similarity).
 MOD_RES 427 427 N6-acetyllysine (By similarity).
 MOD_RES 433 433 Phosphoserine (By similarity).
 MOD_RES 437 437 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Lipid biosynthesis; Lipid metabolism; Mitochondrion; Phosphoprotein; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transferase; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 508 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IDA:RGD.
 GO:0004421; F:hydroxymethylglutaryl-CoA synthase activity; IDA:RGD.
 GO:0060612; P:adipose tissue development; IEP:RGD.
 GO:0007420; P:brain development; IEP:RGD.
 GO:0071230; P:cellular response to amino acid stimulus; IEP:RGD.
 GO:0071398; P:cellular response to fatty acid; IEP:RGD.
 GO:0071385; P:cellular response to glucocorticoid stimulus; IEP:RGD.
 GO:0032869; P:cellular response to insulin stimulus; IEP:RGD.
 GO:0071222; P:cellular response to lipopolysaccharide; IEP:RGD.
 GO:0006695; P:cholesterol biosynthetic process; IEA:UniProtKB-KW.
 GO:0008299; P:isoprenoid biosynthetic process; IEA:InterPro.
 GO:0046951; P:ketone body biosynthetic process; IEP:RGD.
 GO:0001822; P:kidney development; IEP:RGD.
 GO:0001889; P:liver development; IEP:RGD.
 GO:0030324; P:lung development; IEP:RGD.
 GO:0007494; P:midgut development; IEP:RGD.
 GO:0033555; P:multicellular organismal response to stress; IEP:RGD.
 GO:0051591; P:response to cAMP; IEP:RGD.
 GO:0042493; P:response to drug; IEP:RGD.
 GO:0045471; P:response to ethanol; IEP:RGD.
 GO:0033762; P:response to glucagon stimulus; IEP:RGD.
 GO:0060416; P:response to growth hormone stimulus; IEP:RGD.
 GO:0070543; P:response to linoleic acid; IEP:RGD.
 GO:0010038; P:response to metal ion; IEP:RGD.
 GO:0034284; P:response to monosaccharide stimulus; IEP:RGD.
 GO:0007584; P:response to nutrient; IEP:RGD.
 GO:0034696; P:response to prostaglandin F stimulus; IEP:RGD.
 GO:0042594; P:response to starvation; IEP:RGD.
 GO:0009266; P:response to temperature stimulus; IEP:RGD.
 GO:0033574; P:response to testosterone stimulus; IEP:RGD.
 GO:0034014; P:response to triglyceride; IEP:RGD. 

Interpro

 IPR000590; HMG_CoA_synt_AS.
 IPR013746; HMG_CoA_synt_C.
 IPR013528; HMG_CoA_synth_N.
 IPR010122; HMG_CoA_synthase_euk.
 IPR016039; Thiolase-like.
 IPR016038; Thiolase-like_subgr. 

Pfam

 PF08540; HMG_CoA_synt_C
 PF01154; HMG_CoA_synt_N 

SMART

  

PROSITE

 PS01226; HMG_COA_SYNTHASE 

PRINTS