CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022960
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NADH-cytochrome b5 reductase 1 
Protein Synonyms/Alias
 b5R.1; Humb5R2; NAD(P)H:quinone oxidoreductase type 3 polypeptide A2 
Gene Name
 CYB5R1 
Gene Synonyms/Alias
 NQO3A2; UNQ3049/PRO9865 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
81TLGLPVGKHIYLSTRubiquitination[1]
124YLKGVHPKFPEGGKMubiquitination[2, 3]
130PKFPEGGKMSQYLDSubiquitination[4]
139SQYLDSLKVGDVVEFubiquitination[5]
178PEPRVAKKLGMIAGGubiquitination[2, 3]
218LFANQTEKDIILREDubiquitination[5]
248FTLDHPPKDWAYSKGubiquitination[1, 2, 3]
254PKDWAYSKGFVTADMubiquitination[2, 3, 4]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity). 
Sequence Annotation
 DOMAIN 44 156 FAD-binding FR-type.
 NP_BIND 136 166 FAD (By similarity).
 NP_BIND 175 210 FAD (By similarity).  
Keyword
 Complete proteome; Direct protein sequencing; FAD; Flavoprotein; Lipid biosynthesis; Lipid metabolism; Membrane; NAD; Oxidoreductase; Polymorphism; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 305 AA 
Protein Sequence
MGIQTSPVLL ASLGVGLVTL LGLAVGSYLV RRSRRPQVTL LDPNEKYLLR LLDKTTVSHN 60
TKRFRFALPT AHHTLGLPVG KHIYLSTRID GSLVIRPYTP VTSDEDQGYV DLVIKVYLKG 120
VHPKFPEGGK MSQYLDSLKV GDVVEFRGPS GLLTYTGKGH FNIQPNKKSP PEPRVAKKLG 180
MIAGGTGITP MLQLIRAILK VPEDPTQCFL LFANQTEKDI ILREDLEELQ ARYPNRFKLW 240
FTLDHPPKDW AYSKGFVTAD MIREHLPAPG DDVLVLLCGP PPMVQLACHP NLDKLGYSQK 300
MRFTY 305 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0004128; F:cytochrome-b5 reductase activity, acting on NAD(P)H; IEA:EC.
 GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW. 
Interpro
 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
 IPR001834; NADH-Cyt_B5_reductase.
 IPR008333; OxRdtase_FAD-bd_dom.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR017938; Riboflavin_synthase-like_b-brl. 
Pfam
 PF00970; FAD_binding_6
 PF00175; NAD_binding_1 
SMART
  
PROSITE
 PS51384; FAD_FR 
PRINTS
 PR00406; CYTB5RDTASE.
 PR00371; FPNCR.