CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024142
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Methyl-CpG-binding protein 2 
Protein Synonyms/Alias
 MeCp-2 protein; MeCp2 
Gene Name
 Mecp2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
321IEVKEVVKPLLVSTLacetylation[1, 2]
447TTTTVAEKYKHRGEGacetylation[1, 2]
464KDIVSSSMPRPNREEacetylation[3]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] SIRT1-mediated deacetylation of MeCP2 contributes to BDNF expression.
 Zocchi L, Sassone-Corsi P.
 Epigenetics. 2012 Jul;7(7):695-700. [PMID: 22677942] 
Functional Description
 Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (By similarity). 
Sequence Annotation
 DOMAIN 90 162 MBD.
 DNA_BIND 185 197 A.T hook 1.
 DNA_BIND 265 277 A.T hook 2.
 MOD_RES 80 80 Phosphoserine.
 MOD_RES 116 116 Phosphoserine (By similarity).
 MOD_RES 216 216 Phosphoserine (By similarity).
 MOD_RES 229 229 Phosphoserine (By similarity).
 MOD_RES 421 421 Phosphoserine; by CaMK2.
 MOD_RES 424 424 Phosphoserine (By similarity).
 MOD_RES 447 447 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 484 AA 
Protein Sequence
MVAGMLGLRE EKSEDQDLQG LRDKPLKFKK AKKDKKEDKE GKHEPLQPSA HHSAEPAEAG 60
KAETSESSGS APAVPEASAS PKQRRSIIRD RGPMYDDPTL PEGWTRKLKQ RKSGRSAGKY 120
DVYLINPQGK AFRSKVELIA YFEKVGDTSL DPNDFDFTVT GRGSPSRREQ KPPKKPKSPK 180
APGTGRGRGR PKGSGTGRPK AAASEGVQVK RVLEKSPGKL VVKMPFQASP GGKGEGGGAT 240
TSAQVMVIKR PGRKRKAEAD PQAIPKKRGR KPGSVVAAAA AEAKKKAVKE SSIRSVHETV 300
LPIKKRKTRE TVSIEVKEVV KPLLVSTLGE KSGKGLKTCK SPGRKSKESS PKGRSSSASS 360
PPKKEHHHHH HHSESTKAPM PLLPSPPPPE PESSEDPISP PEPQDLSSSI CKEEKMPRGG 420
SLESDGCPKE PAKTQPMVAT TTTVAEKYKH RGEGERKDIV SSSMPRPNRE EPVDSRTPVT 480
ERVS 484 
Gene Ontology
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0000792; C:heterochromatin; IDA:MGI.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0010385; F:double-stranded methylated DNA binding; IDA:MGI.
 GO:0008327; F:methyl-CpG binding; IDA:MGI.
 GO:0003729; F:mRNA binding; IDA:MGI.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:MGI.
 GO:0035197; F:siRNA binding; IDA:MGI.
 GO:0008344; P:adult locomotory behavior; IMP:MGI.
 GO:0001662; P:behavioral fear response; IMP:MGI.
 GO:0032048; P:cardiolipin metabolic process; IMP:MGI.
 GO:0050432; P:catecholamine secretion; IMP:MGI.
 GO:0021549; P:cerebellum development; IMP:MGI.
 GO:0006342; P:chromatin silencing; IMP:MGI.
 GO:0016358; P:dendrite development; IMP:MGI.
 GO:0009790; P:embryo development; IMP:MGI.
 GO:0008211; P:glucocorticoid metabolic process; IMP:MGI.
 GO:0006541; P:glutamine metabolic process; IMP:MGI.
 GO:0016573; P:histone acetylation; IMP:MGI.
 GO:0016571; P:histone methylation; IMP:MGI.
 GO:0006020; P:inositol metabolic process; IMP:MGI.
 GO:0007616; P:long-term memory; IMP:MGI.
 GO:0060291; P:long-term synaptic potentiation; IMP:MGI.
 GO:0006122; P:mitochondrial electron transport, ubiquinol to cytochrome c; IMP:MGI.
 GO:0035067; P:negative regulation of histone acetylation; IMP:MGI.
 GO:0031061; P:negative regulation of histone methylation; IMP:MGI.
 GO:0043524; P:negative regulation of neuron apoptotic process; IMP:MGI.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0001976; P:neurological system process involved in regulation of systemic arterial blood pressure; IMP:MGI.
 GO:0042551; P:neuron maturation; IMP:MGI.
 GO:0009405; P:pathogenesis; IMP:MGI.
 GO:0046470; P:phosphatidylcholine metabolic process; IMP:MGI.
 GO:0008284; P:positive regulation of cell proliferation; IMP:MGI.
 GO:0051965; P:positive regulation of synapse assembly; IMP:MGI.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:MGI.
 GO:0009791; P:post-embryonic development; IMP:MGI.
 GO:0019230; P:proprioception; IMP:MGI.
 GO:0008104; P:protein localization; IMP:MGI.
 GO:0060079; P:regulation of excitatory postsynaptic membrane potential; IMP:MGI.
 GO:0006349; P:regulation of gene expression by genetic imprinting; IMP:MGI.
 GO:0002087; P:regulation of respiratory gaseous exchange by neurological system process; IMP:MGI.
 GO:0007585; P:respiratory gaseous exchange; IMP:MGI.
 GO:0001666; P:response to hypoxia; IMP:MGI.
 GO:0019233; P:sensory perception of pain; IMP:MGI.
 GO:0035176; P:social behavior; IMP:MGI.
 GO:0001964; P:startle response; IMP:MGI.
 GO:0007416; P:synapse assembly; IMP:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0021591; P:ventricular system development; IMP:MGI.
 GO:0008542; P:visual learning; IMP:MGI. 
Interpro
 IPR017956; AT_hook_DNA-bd_motif.
 IPR016177; DNA-bd_integrase-typ.
 IPR017353; Me_CpG-bd_MeCP2.
 IPR001739; Methyl_CpG_DNA-bd. 
Pfam
 PF01429; MBD 
SMART
 SM00384; AT_hook
 SM00391; MBD 
PROSITE
 PS50982; MBD 
PRINTS