CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024609
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ryanodine receptor 2 
Protein Synonyms/Alias
 RYR-2; RyR2; Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor-calcium release channel; Type 2 ryanodine receptor 
Gene Name
 Ryr2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
167KQRSEGEKVRVGDDLacetylation[1]
1134AFAFDGFKAQRWHQGacetylation[1]
3287IDEGAWMKRLAVFSQubiquitination[2]
3461SLIVAALKRLLPIGLacetylation[1]
3567SKKAVWHKLLSKQRKacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development (By similarity). 
Sequence Annotation
 DOMAIN 103 158 MIR 1.
 DOMAIN 165 210 MIR 2.
 DOMAIN 218 273 MIR 3.
 DOMAIN 279 336 MIR 4.
 DOMAIN 344 401 MIR 5.
 DOMAIN 592 802 B30.2/SPRY 1.
 REPEAT 846 959 1.
 REPEAT 960 1073 2.
 DOMAIN 1018 1215 B30.2/SPRY 2.
 DOMAIN 1350 1556 B30.2/SPRY 3.
 REPEAT 2682 2800 3.
 REPEAT 2802 2915 4.
 REGION 846 2915 4 X approximate repeats.
 REGION 3559 3588 Interaction with CALM (By similarity).
 MOD_RES 2021 2021 Phosphoserine; by PKA (By similarity).
 MOD_RES 2798 2798 Phosphoserine; by CaMK2D and PKA.
 MOD_RES 2804 2804 Phosphoserine; by CaMK2D.  
Keyword
 3D-structure; Alternative splicing; Calcium; Calcium channel; Calcium transport; Calmodulin-binding; Complete proteome; Developmental protein; Ion channel; Ion transport; Ligand-gated ion channel; Membrane; Phosphoprotein; Receptor; Reference proteome; Repeat; Sarcoplasmic reticulum; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 4953 AA 
Protein Sequence
MADAGEGEDE IQFLRTDDEV VLQCTATIHK EQQKLCLAAE GFGNRLCFLE STSNSKNVPP 60
DLSICTFVLE QSLSVRALQE MLANTVEKSE GKFMMKTAQG GGHRTLLYGH AILLRHSYSG 120
MYLCCLSTSR SSTDKLAFDV GLQEDTTGEA CWWTIHPASK QRSEGEKVRV GDDLILVSVS 180
SERYLHLSYG NSSWRVDAAF QQTLWSVAPI SSGSEAAQGY LIGGDVLRLL HGHMDECLTV 240
PSGEHGEEQR RTVHYEGGAV SVHARSLWRL ETLRVAWSGS HIRWGQPFRL RHVTTGKYLS 300
LMEDKNLLLM DKEKADVKST AFAFRSSKEK LDAGVRKEVD GMGTSEIKYG DSICYIQHVD 360
TGLWLTYQAV DVKSARMGSI QRKAIMHHEG HMDDGLNLSR SQHEESRTAR VIRSTVFLFN 420
RFIRGLDALS KRAKLPTVDL PIESVSLSLQ DLIGYFHPPD EHLEHEDKQN RLRALKNRQN 480
LFQEEGMINL VLECIDRLHV YSSAAHFADV AGREAGESWK SILNSLYELL AALIRGNRKN 540
CAQFSGSLDW LISRLERLEA SSGILEVLHC VLVESPEALN IIKEGHIKSI ISLLDKHGRN 600
HKVLDVLCSL CVCHGVAVRS NQHLICDNLL PGRDLLLQTR LVNHVSSMRP NIFLGVSEGS 660
AQYKKWYYEL MVDHTEPFVT AEATHLRVGW ASTEGYSPYP GGGEEWGGNG VGDDLFSYGF 720
DGLHLWSGCI ARTVSSPNQH LLRTDDVISC CLDLSAPSIS FRINGQPVQG MFENFNIDGL 780
FFPVVSFSAG IKVRFLLGGR HGEFKFLPPP GYAACYEAVL PKEKLKVEHS REYKQERTYT 840
RDLLGPTVSL TQAAFTPVPV DTSQIVLPPH LERIRERLAE NIHELWVMNK IELGWQYGPV 900
RDDNKRQHPC LVEFCKLPEQ ERNYNLQMSL ETLKTLLALG CHVGIADEHA EEKVKKMKLP 960
KNYQLTSGYK PAPMDLSFIK LTPSQEAMVD KLAENAHNVW ARDRIRQGWT YGIQQDVKNR 1020
RNPRLVPYTL LDDRTKKSNK DSLREAVRTL LGYGYHLEAP DQDHASRAEV CSGTGERFRI 1080
FRAEKTYAVK AGRWYFEFEA VTAGDMRVGW SRPGCQPDLE LGSDERAFAF DGFKAQRWHQ 1140
GNEHYGRSWQ AGDVVGCMVD MNEHTMMFTL NGEILLDDSG SELAFKDFDV GDGFIPVCSL 1200
GVAQVGRMNF GKDVSTLKYF TICGLQEGYE PFAVNTNRDI TMWLSKRLPQ FLQVPSNHEH 1260
IEVTRIDGTI DSSPCLKVTQ KSFGSQNSNT DIMFYRLSMP IECAEVFSKS VAGGIPGAGF 1320
YGPKNDLEDF DVDSDFEVLM KTAHGHLVPD RMDKDKETPK PEFNNHKDYA QEKPSRLKQR 1380
FLLRRTKPDY STSHSARLTE DVLADDRDDY EYLMQTSTYY YSVRIFPGQE PANVWVGWIT 1440
SDFHQYDTGF DLDRVRTVTV TLGDEKGKVH ESIKRSNCYM VCAGESMSPG QGRNNSNGLE 1500
IGCVVDAASG LLTFIANGKE LSTYYQVEPS TKLFPAVFAQ ATSPNVFQFE LGRIKNVMPL 1560
SAGLFKSEHK NPVPQCPPRL HVQFLSHVLW SRMPNQFLKV DVSRISERQG WLVQCLDPLQ 1620
FMSLHIPEEN RSVDILELTE QEELLQFHYH TLRLYSAVCA LGNHRVAHAL CSHVDEPQLL 1680
YAIENKYMPG LLRAGYYDLL IDIHLSSYAT ARLMMNNEFI VPMTEETKSI TLFPDENKKH 1740
GLPGIGLSTS LRPRMCFSSP SFVSISNECY QYSPEFPLDI LKAKTIQMLT EAVKEGSLHA 1800
RDPVGGTTEF LFVPLIKLFY TLLIMGIFHN EDLKHILQLI EPSVFKEAAT PEEEGGAPEK 1860
EISIDDSKLE VKEEAKAGKR PKEGLLQMKL PEPVKLQMCL LLQYLCDCQV RHRIEAIVAF 1920
SDDFVAKLQD NQRFRYNEVM QALNMSAALT ARKEFRSPPQ EQINMLLNFK DDKSECPCPE 1980
EIRDQLLDFH EDLMTHCGIE LDEDGSLDGS NDLTIRGRLL SLVEKVTYLK KKQAEKPVAS 2040
DSRKSSSLQQ LISETMVRWA QESVIEDPEL VRAMFVLLHR QYDGIGGLVR ALPKTYTING 2100
VSVEDTINLL ASLGQIRSLL SVRMGKEEEK LMIRGLGDIM NNKVFYQHPN LMRALGMHET 2160
VMEVMVNVLG GGESKEITFP KMVANCCRFL CYFCRISRQN QKAMFDHLSY LLENSSVGLA 2220
SPAMRGSTPL DVAAASVMDN NELALALREP DLEKVVRYLA GCGLQSCQML VSKGYPDIGW 2280
NPVEGERYLD FLRFAVFCNG ESVEENANVV VRLLIRRPEC FGPALRGEGG NGLLAAMEEA 2340
IKIAEDPSRD GPSPTSGSSK TLDAEEEEDD TIHMGNAIMT FYAALIDLLG RCAPEMHLIH 2400
AGKGEAIRIR SILRSLIPLG DLVGVISIAF QMPTIAKDGK VVEPDMSAGF CPDHKAAMVL 2460
FLDRVYGIEV QDFLLHLLEV GFLPDLRAAA SLDTAALSAT DMALALNRYL CTAVLPLLTR 2520
CAPLFAGTEH HASLIDSLLH TVYRLSKGCS LTKAQRDSIE VCLLSICGQL RPSMMQHLLR 2580
RLVFDVPLLN EHAKMPLKLL TNHYERCWKY YCLPGGWSNF GAASEEELHL SRKLFWGIFD 2640
ALSQKKYEQE LFKLALPCLS AVAGALPPDY MESNYVSMME KQSSMDSEGN FNPQPVDTSN 2700
ITIPEKLEYF INKYAEHSHD KWSMDKLANG WIYGEIYSDS SKIQPLMKPY KLLSEKEKEI 2760
YRWPIKESLK TMLAWGWRIE RTREGDSMAL YNRTRRISQT SQVSIDAAHG YSPRAIDMSN 2820
VTLSRDLHAM AEMMAENYHN IWAKKKKMEL ESKGGGNHPL LVPYDTLTAK EKAKDREKAQ 2880
DIFKFLQISG YAVSRGFKDL DLDTPSIEKR FAYSFLQQLI RYVDEAHQYI LEFDGGSRSK 2940
GEHFPYEQEI KFFAKVVLPL IDQYFKNHRL YFLSAASRPL CTGGHASNKE KEMVTSLFCK 3000
LGVLVRHRIS LFGNDATSIV NCLHILGQTL DARTVMKTGL DSVKSALRAF LDNAAEDLEK 3060
TMENLKQGQF THTRSQPKGV TQIINYTTVA LLPMLSSLFE HIGQHQFGED LILEDVQVSC 3120
YRILTSLYAL GTSKSIYVER QRSALGECLA AFAGAFPIAF LETHLDKHNV YSIYNTRSSR 3180
ERAALSLPAN VEDVCPNIPS LEKLMTEIIE LAESGIRYTQ MPHMMEVVLP MLCSYMSRWW 3240
EHGPENHPER AEMCCTALNS EHMNTLLGNI LKIIYNNLGI DEGAWMKRLA VFSQPIINKV 3300
KPQLLKTHFL PLMEKLKKKA AMVVSEEDHL KAEARGDMSE AELLILDEFT TLARDLYAFY 3360
PLLIRFVDYN RAKWLKEPNP EAEELFRMVA EVFIYWSKSH NFKREEQNFV VQNEINNMSF 3420
LITDTKSKMS KAAISDQERK KMKRKGDRYS MQTSLIVAAL KRLLPIGLNI CAPGDQELIA 3480
LAKNRFSLKD TEEEVRDVIR SNIHLQGKLE DPAIRWQMAL YKDLPNRAED TSDPERTVER 3540
VLDIANVLFH LEQVEHPQRS KKAVWHKLLS KQRKRAVVAC FRMAPLYNLP RHRAVNLFLQ 3600
GYEKSWIETE EHYFEDKLIE DLAKPGSELP EEDEAMKRVD PLHQLILLFS RTALTEKCKL 3660
EEDFLYMAYA DIMAKSCHDE EDDDGEEEVK SFEVTGSQRS KEKEMEKQKL LYQQARLHDR 3720
GAAEMVLQTF SASKGETGPM VAATLKLGIA ILNGGNSTVQ QKMLDYLKEK KDVGFFQSLA 3780
GLMQSCSVLD LNAFERQNKA EGLGMVTEEG SGEKVLQDDE FTCDLFRFLQ LLCEGHNSDF 3840
QNYLRTQTGN NTTVNIIIST VDYLLRVQES ISDFYWYYSG KDIIDEQGQR NFSKAIQVAK 3900
QVFNTLTEYI QGPCTGNQQS LAHSRLWDAV VGFLHVFAHM QMKLSQDSSQ IELLKELMDL 3960
QKDMVVMLLS MLEGNVVNGT IGKQMVDMLV ESSNNVEMIL KFFDMFLKLK DLTSSDTFKE 4020
YDPDGKGVIS KRDFHKAMES HKHYTQSETE FLLSCAETDE NETLDYEEFV KRFHEPAKDI 4080
GFNVAVLLTN LSEHMPNDTR LQTFLELAES VLNYFQPFLG RIEIMGSAKR IERVYFEISE 4140
SSRTQWEKPQ VKESKRQFIF DVVNEGGEKE KMELFVNFCE DTIFEMQLAA QISESDLNER 4200
SANKEESEKE RPEEQAPRMG FFSLLTVQSA LFALRYNVLT LVRMLSLKSL KKQMKRMKKM 4260
TVKDMVSAFF SSYWSVFVTL LHFVASVCRG FFRIVSSLLL GGSLVEGAKK IKVAELLANM 4320
PDPTQDEVRG DEEEGERKPL ESALPSEDLT DLKELTEESD LLSDIFGLDL KREGGQYKLI 4380
PHNPNAGLSD LMTNPIPVPE VQEKFQEQKV KEEKEGKEET KSEPEKAEGE DGEKEEKAKD 4440
DKGKQKLRQL HTHRYGEPEV PESAFWKKII AYQQKLLNYF ARNFYNMRML ALFVAFAINF 4500
ILLFYKVSTS SVVEGKELPT RTSSDAAKVT TSLDSSPHRI IAVHYVLEES SGYMEPTLRI 4560
LAILHTIISF FCIIGYYCLK VPLVIFKREK EVARKLEFDG LYITEQPSED DIKGQWDRLV 4620
INTQSFPNNY WDKFVKRKVM DKYGEFYGRD RISELLGMDK AALDFSDARE KKKPKKDSSL 4680
SAVLNSIDVK YQMWKLGVVF TDNSFLYLAW YMTMSVLGHY NNFFFAAHLL DIAMGFKTLR 4740
TILSSVTHNG KQLVLTVGLL AVVVYLYTVV AFNFFRKFYN KSEDGDTPDM KCDDMLTCYM 4800
FHMYVGVRAG GGIGDEIEDP AGDEYEIYRI IFDITFFFFV IVILLAIIQG LIIDAFGELR 4860
DQQEQVKEDM ETKCFICGIG NDYFDTVPHG FETHTLQEHN LANYLFFLMY LINKDETEHT 4920
GQESYVWKMY QERCWEFFPA GDCFRKQYED QLN 4953 
Gene Ontology
 GO:0031672; C:A band; IDA:RGD.
 GO:0031234; C:extrinsic to internal side of plasma membrane; IDA:RGD.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0043005; C:neuron projection; IDA:RGD.
 GO:0043234; C:protein complex; IDA:RGD.
 GO:0042383; C:sarcolemma; IDA:RGD.
 GO:0033017; C:sarcoplasmic reticulum membrane; IDA:BHF-UCL.
 GO:0030018; C:Z disc; IDA:BHF-UCL.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0048763; F:calcium-induced calcium release activity; IDA:RGD.
 GO:0005219; F:ryanodine-sensitive calcium-release channel activity; IDA:RGD.
 GO:0019722; P:calcium-mediated signaling; ISS:UniProtKB.
 GO:0071313; P:cellular response to caffeine; ISS:UniProtKB.
 GO:0003143; P:embryonic heart tube morphogenesis; ISS:UniProtKB.
 GO:0071421; P:manganese ion transmembrane transport; IMP:RGD.
 GO:0051481; P:reduction of cytosolic calcium ion concentration; IDA:RGD.
 GO:0051592; P:response to calcium ion; IDA:RGD.
 GO:0042493; P:response to drug; IEP:RGD.
 GO:0032026; P:response to magnesium ion; IDA:RGD.
 GO:0007584; P:response to nutrient; IEP:RGD.
 GO:0070296; P:sarcoplasmic reticulum calcium ion transport; IMP:RGD. 
Interpro
 IPR001870; B30.2/SPRY.
 IPR000699; Ca-rel_channel.
 IPR008985; ConA-like_lec_gl_sf.
 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom.
 IPR014821; Ins145_P3_rcpt.
 IPR005821; Ion_trans_dom.
 IPR016093; MIR_motif.
 IPR013662; RIH_assoc-dom.
 IPR013333; Ryan_recept.
 IPR003032; Ryanodine_rcpt.
 IPR015925; Ryanodine_recept-rel.
 IPR009460; Ryanrecept_TM4-6.
 IPR018355; SPla/RYanodine_receptor_subgr.
 IPR003877; SPRY_rcpt. 
Pfam
 PF08709; Ins145_P3_rec
 PF00520; Ion_trans
 PF02815; MIR
 PF08454; RIH_assoc
 PF06459; RR_TM4-6
 PF01365; RYDR_ITPR
 PF02026; RyR
 PF00622; SPRY 
SMART
 SM00054; EFh
 SM00472; MIR
 SM00449; SPRY 
PROSITE
 PS50188; B302_SPRY
 PS50919; MIR 
PRINTS
 PR00795; RYANODINER.