UniProt Accession

 RTCA_HUMAN; O00442; Q5VVL5; Q5VVL6; Q96E99 

Genbank Protein ID

 Y11651; Y11652; AL445928; AL663111; AL445928; AL663111; AL663111; AL445928; AL663111; AL445928; CH471097; CH471097; BC012604 

Genbank Nucleotide ID

 CAA72364.1; CAA72365.1; CAH72259.1; CAH72259.1; CAH72260.1; CAH72260.1; CAH73852.1; CAH73852.1; CAH73853.1; CAH73853.1; EAW72961.1; EAW72962.1; AAH12604.1 

Protein Name

 RNA 3'-terminal phosphate cyclase 

Protein Synonyms/Alias

 RNA cyclase; RNA-3'-phosphate cyclase; RNA terminal phosphate cyclase domain-containing protein 1; RTC domain-containing protein 1 

Gene Name

 RTCA 

Gene Synonyms/Alias

 RPC; RPC1; RTC1; RTCD1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
154	DYTVMVFKPIVEKFG	ubiquitination	[1]
169	FIFNCDIKTRGYYPK	ubiquitination	[1]
176	KTRGYYPKGGGEVIV	ubiquitination	[1, 2]
189	IVRMSPVKQLNPINL	ubiquitination	[1]
204	TERGCVTKIYGRAFV	ubiquitination	[1]
221	VLPFKVAKDMAAAAV	ubiquitination	[1]
285	KRGVNADKVGIEAAE	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'- phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. 

Sequence Annotation

 NP_BIND 294 298 ATP (By similarity).
 ACT_SITE 320 320 Tele-AMP-histidine intermediate (By
 BINDING 104 104 ATP (By similarity).  

Keyword

 Alternative splicing; ATP-binding; Complete proteome; Direct protein sequencing; Ligase; Nucleotide-binding; Nucleus; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 366 AA 

Protein Sequence

Gene Ontology

 GO:0005654; C:nucleoplasm; TAS:ProtInc.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; TAS:ProtInc.
 GO:0003963; F:RNA-3'-phosphate cyclase activity; TAS:ProtInc.
 GO:0006396; P:RNA processing; IEA:InterPro. 

Interpro

 IPR013791; RNA3'-term_phos_cycl_insert.
 IPR023797; RNA3'_phos_cyclase_dom.
 IPR000228; RNA3'_term_phos_cyc.
 IPR017770; RNA3'_term_phos_cyc_type_1.
 IPR020719; RNA3'_term_phos_cycl-like_CS.
 IPR013792; RNA3'P_cycl/enolpyr_Trfase_a/b. 

Pfam

 PF01137; RTC
 PF05189; RTC_insert 

SMART

  

PROSITE

 PS01287; RTC 

PRINTS