UniProt Accession

 ACOT8_HUMAN; O14734; O15261; Q17RX4 

Genbank Protein ID

 AF014404; X86032; AF124264; AL008726; BC117155; BC117157 

Genbank Nucleotide ID

 AAB71665.1; CAA60024.1; AAD27616.1; CAA15502.1; AAI17156.1; AAI17158.1 

Protein Name

 Acyl-coenzyme A thioesterase 8 

Protein Synonyms/Alias

 Acyl-CoA thioesterase 8; Choloyl-coenzyme A thioesterase; HIV-Nef-associated acyl-CoA thioesterase; PTE-2; Peroxisomal acyl-coenzyme A thioester hydrolase 1; PTE-1; Peroxisomal long-chain acyl-CoA thioesterase 1; Thioesterase II; hACTE-III; hACTEIII; hTE 

Gene Name

 ACOT8 

Gene Synonyms/Alias

 ACTEIII; PTE1; PTE2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
52	RHYWVPAKRLFGGQI	acetylation	[1]
52	RHYWVPAKRLFGGQI	ubiquitination	[2]
188	QEVPIEIKPVNPSPL	ubiquitination	[2]
318	KPQVSESKL******	acetylation	[1]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N- acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (By similarity). May be involved in the metabolic regulation of peroxisome proliferation. 

Sequence Annotation

 MOTIF 317 319 Microbody targeting signal (Potential).
 ACT_SITE 232 232 Charge relay system (By similarity).
 ACT_SITE 254 254 Charge relay system (By similarity).
 ACT_SITE 304 304 Charge relay system (By similarity).
 MOD_RES 2 2 Phosphoserine (By similarity).  

Keyword

 Complete proteome; Host-virus interaction; Hydrolase; Peroxisome; Peroxisome biogenesis; Phosphoprotein; Reference proteome; Serine esterase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 319 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005782; C:peroxisomal matrix; IDA:UniProtKB.
 GO:0004091; F:carboxylesterase activity; IEA:UniProtKB-KW.
 GO:0033882; F:choloyl-CoA hydrolase activity; IEA:EC.
 GO:0052815; F:medium-chain acyl-CoA hydrolase activity; IDA:UniProtKB.
 GO:0016290; F:palmitoyl-CoA hydrolase activity; IDA:UniProtKB.
 GO:0006637; P:acyl-CoA metabolic process; IDA:UniProtKB.
 GO:0036109; P:alpha-linolenic acid metabolic process; TAS:Reactome.
 GO:0006699; P:bile acid biosynthetic process; TAS:Reactome.
 GO:0043649; P:dicarboxylic acid catabolic process; IDA:UniProtKB.
 GO:0033540; P:fatty acid beta-oxidation using acyl-CoA oxidase; TAS:Reactome.
 GO:0016559; P:peroxisome fission; IDA:UniProtKB.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR003703; Acyl_CoA_thio. 

Pfam

  

SMART

  

PROSITE

  

PRINTS