UniProt Accession

 UBP21_HUMAN; Q9UK80; Q59H60; Q5BKT5; Q5VTW9; Q5VTX0; Q9BTV1; Q9HBS2; Q9NYN4 

Genbank Protein ID

 AF177758; AF233442; AK292319; AF217979; AB208899; AL590714; AL590714; CH471121; BC003130; BC090946; BC136291 

Genbank Nucleotide ID

 AAD54321.1; AAF61308.1; BAF85008.1; AAG17222.1; BAD92136.1; CAH72142.1; CAH72143.1; EAW52644.1; AAH03130.2; AAH90946.1; AAI36292.1 

Protein Name

 Ubiquitin carboxyl-terminal hydrolase 21 

Protein Synonyms/Alias

 Deubiquitinating enzyme 21; Ubiquitin thioesterase 21; Ubiquitin-specific-processing protease 21 

Gene Name

 USP21 

Gene Synonyms/Alias

 USP23; PP1490 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
289	RFRAVFQKYVPSFSG	ubiquitination	[1]
379	DLFVGQLKSCLKCQA	ubiquitination	[1]
407	LSLPIPKKGFAGGKV	ubiquitination	[2]
413	KKGFAGGKVSLRDCF	ubiquitination	[2]
475	ASRGSIKKSSVGVDF	ubiquitination	[1, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination (By similarity). Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates. 

Sequence Annotation

 MOTIF 134 152 Nuclear export signal.
 ACT_SITE 221 221 Nucleophile.
 ACT_SITE 518 518 Proton acceptor (By similarity).
 METAL 384 384 Zinc.
 METAL 387 387 Zinc.
 METAL 437 437 Zinc.
 METAL 440 440 Zinc.  

Keyword

 3D-structure; Activator; Alternative splicing; Chromatin regulator; Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Nucleus; Polymorphism; Protease; Reference proteome; Thiol protease; Transcription; Transcription regulation; Ubl conjugation pathway; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 565 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0019784; F:NEDD8-specific protease activity; IDA:UniProtKB.
 GO:0003713; F:transcription coactivator activity; ISS:UniProtKB.
 GO:0004221; F:ubiquitin thiolesterase activity; IDA:UniProtKB.
 GO:0004843; F:ubiquitin-specific protease activity; IDA:UniProtKB.
 GO:0016578; P:histone deubiquitination; ISS:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 

Interpro

 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19. 

Pfam

 PF00443; UCH 

SMART

  

PROSITE

 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 

PRINTS