CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030534
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 3 subunit H 
Protein Synonyms/Alias
 eIF3h; Eukaryotic translation initiation factor 3 subunit 3; eIF-3 gamma; eIF3 p40 subunit 
Gene Name
 EIF3S3 
Gene Synonyms/Alias
 EIF3H; hCG_20900 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19GKKMASRKEGTGSTAubiquitination[1, 2, 3, 4]
38STAGAAGKGKGKGGSubiquitination[1, 2, 4, 5]
42AAGKGKGKGGSGDSAubiquitination[1]
51GSGDSAVKQVQIDGLubiquitination[1]
179AQGSLSLKAYRLTPKubiquitination[1, 2, 6]
186KAYRLTPKLMEVCKEubiquitination[2]
192PKLMEVCKEKDFSPEubiquitination[2, 4, 6]
194LMEVCKEKDFSPEALubiquitination[2, 4]
202DFSPEALKKANITFEubiquitination[1, 3, 5]
203FSPEALKKANITFEYubiquitination[2, 4, 6]
234VLMWELEKKSAVADKubiquitination[1, 2, 6]
235LMWELEKKSAVADKHubiquitination[2, 4]
241KKSAVADKHELLSLAacetylation[4]
241KKSAVADKHELLSLAubiquitination[1, 2, 3, 4, 6, 7, 8, 9]
255ASSNHLGKNLQLLMDubiquitination[1, 6]
273EMSQDIVKYNTYMRNubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9]
288TSKQQQQKHQYQQRRubiquitination[1]
317LPEEDLSKLFKPPQPubiquitination[2, 4, 7, 9]
320EDLSKLFKPPQPPARubiquitination[1, 2, 4, 5, 7, 9]
345NTYCQNIKEFTAQNLubiquitination[1, 6]
354FTAQNLGKLFMAQALubiquitination[1, 2, 3, 4, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [9] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (By similarity). 
Sequence Annotation
 DOMAIN 48 159 MPN (By similarity).
 MOD_RES 197 197 Phosphoserine (By similarity).  
Keyword
 Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 366 AA 
Protein Sequence
MYSRHAQRIP PGKKMASRKE GTGSTATSSS STAGAAGKGK GKGGSGDSAV KQVQIDGLVV 60
LKIIKHYQEE GQGTEVVQGV LLGLVVEDRL EITNCFPFPQ HTEDDADFDE VQYQMEMMRS 120
LRHVNIDHLH VGWYQSTYYG SFVTRALLDS QFSYQHAIEE SVVLIYDPIK TAQGSLSLKA 180
YRLTPKLMEV CKEKDFSPEA LKKANITFEY MFEEVPIVIK NSHLINVLMW ELEKKSAVAD 240
KHELLSLASS NHLGKNLQLL MDRVDEMSQD IVKYNTYMRN TSKQQQQKHQ YQQRRQQENM 300
QRQSRGEPPL PEEDLSKLFK PPQPPARMDS LLIAGQINTY CQNIKEFTAQ NLGKLFMAQA 360
LQEYNN 366 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IEA:HAMAP.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP. 
Interpro
 IPR027524; eIF3h.
 IPR000555; JAB1_Mov34_MPN_PAD1. 
Pfam
 PF01398; JAB 
SMART
 SM00232; JAB_MPN 
PROSITE
  
PRINTS