CPLM-008714

Genbank Nucleotide ID

Geranylgeranyl transferase type-2 subunit beta

Protein Synonyms/Alias

Geranylgeranyl transferase type II subunit beta; GGTase-II-beta; Rab geranyl-geranyltransferase subunit beta; Rab GG transferase beta; Rab GGTase beta; Rab geranylgeranyltransferase subunit beta; Type II protein geranyl-geranyltransferase subunit beta

Homo sapiens (Human)

Position	Peptide	Type	References
6	**MGTPQKDVIIKSD	acetylation	[1]
6	**MGTPQKDVIIKSD	ubiquitination	[1, 2, 3, 4]
11	PQKDVIIKSDAPDTL	ubiquitination	[4, 5, 6, 7]
22	PDTLLLEKHADYIAS	ubiquitination	[4, 5, 6, 7]
33	YIASYGSKKDDYEYC	ubiquitination	[5, 6]
34	IASYGSKKDDYEYCM	ubiquitination	[6]
124	NKVVEYVKGLQKEDG	ubiquitination	[3, 5, 6, 7]

[1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[6] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to both cysteines in Rab proteins with an -XXCC, -XCXC and -CCXX C-terminal, such as RAB1A, RAB3A and RAB5A respectively.

REPEAT 20 61 PFTB 1.
REPEAT 68 109 PFTB 2.
REPEAT 116 157 PFTB 3.
REPEAT 164 205 PFTB 4.
REPEAT 212 253 PFTB 5.
REPEAT 260 302 PFTB 6.
METAL 238 238 Zinc (By similarity).
METAL 240 240 Zinc (By similarity).
METAL 290 290 Zinc (By similarity).
MOD_RES 2 2 N-acetylglycine.
MOD_RES 3 3 Phosphothreonine.

Acetylation; Complete proteome; Metal-binding; Phosphoprotein; Prenyltransferase; Reference proteome; Repeat; Transferase; Zinc.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0004663; F:Rab geranylgeranyltransferase activity; IEA:EC.
GO:0006464; P:cellular protein modification process; NAS:UniProtKB.
GO:0018344; P:protein geranylgeranylation; IEA:InterPro.
GO:0007601; P:visual perception; TAS:ProtInc.

IPR001330; Prenyltrans.
IPR026873; Ptb1.
IPR008930; Terpenoid_cyclase/PrenylTrfase.

PF00432; Prenyltrans