CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000708
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 4 gamma 3 
Protein Synonyms/Alias
 eIF-4-gamma 3; eIF-4G 3; eIF4G 3; eIF-4-gamma II; eIF4GII 
Gene Name
 EIF4G3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
901KFIGELFKLKMLTEAubiquitination[1]
944GKDLDFEKAKPRMDQacetylation[2]
1069EKIQLVPKAQLGSWGubiquitination[3]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1. 
Sequence Annotation
 REPEAT 745 783 HEAT 1.
 DOMAIN 755 983 MIF4G.
 REPEAT 784 831 HEAT 2.
 REPEAT 832 905 HEAT 3.
 REPEAT 906 944 HEAT 4.
 REPEAT 945 984 HEAT 5.
 DOMAIN 1221 1343 MI.
 DOMAIN 1416 1585 W2.
 REGION 134 162 PABPC1-binding.
 REGION 619 630 EIF4E-binding (By similarity).
 REGION 699 1019 eIF3/EIF4A-binding (By similarity).
 REGION 1433 1585 EIF4A-binding (By similarity).
 REGION 1571 1585 Necessary but not sufficient for MKNK1-
 MOD_RES 230 230 Phosphoserine.
 MOD_RES 232 232 Phosphoserine.
 MOD_RES 240 240 Phosphoserine (By similarity).
 MOD_RES 267 267 Phosphoserine (By similarity).
 MOD_RES 495 495 Phosphoserine.
 MOD_RES 1156 1156 Phosphoserine; by CaMK1.  
Keyword
 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Host-virus interaction; Initiation factor; Phosphoprotein; Polymorphism; Protein biosynthesis; Reference proteome; Repeat; RNA-binding; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1585 AA 
Protein Sequence
MNSQPQTRSP FFQRPQIQPP RATIPNSSPS IRPGAQTPTA VYQANQHIMM VNHLPMPYPV 60
PQGPQYCIPQ YRHSGPPYVG PPQQYPVQPP GPGPFYPGPG PGDFPNAYGT PFYPSQPVYQ 120
SAPIIVPTQQ QPPPAKREKK TIRIRDPNQG GKDITEEIMS GGGSRNPTPP IGRPTSTPTP 180
PQQLPSQVPE HSPVVYGTVE SAHLAASTPV TAASDQKQEE KPKPDPVLKS PSPVLRLVLS 240
GEKKEQEGQT SETTAIVSIA ELPLPPSPTT VSSVARSTIA APTSSALSSQ PIFTTAIDDR 300
CELSSPREDT IPIPSLTSCT ETSDPLPTNE NDDDICKKPC SVAPNDIPLV SSTNLINEIN 360
GVSEKLSATE SIVEIVKQEV LPLTLELEIL ENPPEEMKLE CIPAPITPST VPSFPPTPPT 420
PPASPPHTPV IVPAAATTVS SPSAAITVQR VLEEDESIRT CLSEDAKEIQ NKIEVEADGQ 480
TEEILDSQNL NSRRSPVPAQ IAITVPKTWK KPKDRTRTTE EMLEAELELK AEEELSIDKV 540
LESEQDKMSQ GFHPERDPSD LKKVKAVEEN GEEAEPVRNG AESVSEGEGI DANSGSTDSS 600
GDGVTFPFKP ESWKPTDTEG KKQYDREFLL DFQFMPACIQ KPEGLPPISD VVLDKINQPK 660
LPMRTLDPRI LPRGPDFTPA FADFGRQTPG GRGVPLLNVG SRRSQPGQRR EPRKIITVSV 720
KEDVHLKKAE NAWKPSQKRD SQADDPENIK TQELFRKVRS ILNKLTPQMF NQLMKQVSGL 780
TVDTEERLKG VIDLVFEKAI DEPSFSVAYA NMCRCLVTLK VPMADKPGNT VNFRKLLLNR 840
CQKEFEKDKA DDDVFEKKQK ELEAASAPEE RTRLHDELEE AKDKARRRSI GNIKFIGELF 900
KLKMLTEAIM HDCVVKLLKN HDEESLECLC RLLTTIGKDL DFEKAKPRMD QYFNQMEKIV 960
KERKTSSRIR FMLQDVIDLR LCNWVSRRAD QGPKTIEQIH KEAKIEEQEE QRKVQQLMTK 1020
EKRRPGVQRV DEGGWNTVQG AKNSRVLDPS KFLKITKPTI DEKIQLVPKA QLGSWGKGSS 1080
GGAKASETDA LRSSASSLNR FSALQPPAPS GSTPSTPVEF DSRRTLTSRG SMGREKNDKP 1140
LPSATARPNT FMRGGSSKDL LDNQSQEEQR REMLETVKQL TGGVDVERNS TEAERNKTRE 1200
SAKPEISAMS AHDKAALSEE ELERKSKSII DEFLHINDFK EAMQCVEELN AQGLLHVFVR 1260
VGVESTLERS QITRDHMGQL LYQLVQSEKL SKQDFFKGFS ETLELADDMA IDIPHIWLYL 1320
AELVTPMLKE GGISMRELTI EFSKPLLPVG RAGVLLSEIL HLLCKQMSHK KVGALWREAD 1380
LSWKDFLPEG EDVHNFLLEQ KLDFIESDSP CSSEALSKKE LSAEELYKRL EKLIIEDKAN 1440
DEQIFDWVEA NLDEIQMSSP TFLRALMTAV CKAAIIADSS TFRVDTAVIK QRVPILLKYL 1500
DSDTEKELQA LYALQASIVK LDQPANLLRM FFDCLYDEEV ISEDAFYKWE SSKDPAEQNG 1560
KGVALKSVTA FFTWLREAEE ESEDN 1585 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016281; C:eukaryotic translation initiation factor 4F complex; TAS:ProtInc.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0000339; F:RNA cap binding; TAS:ProtInc.
 GO:0008135; F:translation factor activity, nucleic acid binding; TAS:ProtInc.
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0006446; P:regulation of translational initiation; TAS:ProtInc.
 GO:0016070; P:RNA metabolic process; IEA:InterPro.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR016024; ARM-type_fold.
 IPR003891; Initiation_fac_eIF4g_MI.
 IPR016021; MIF4-like_typ_1/2/3.
 IPR003890; MIF4G-like_typ-3.
 IPR003307; W2_domain. 
Pfam
 PF02847; MA3
 PF02854; MIF4G
 PF02020; W2 
SMART
 SM00515; eIF5C
 SM00544; MA3
 SM00543; MIF4G 
PROSITE
 PS50077; HEAT_REPEAT
 PS51366; MI
 PS51363; W2 
PRINTS