CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002704
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Guanine nucleotide-binding protein G(i) subunit alpha-2 
Protein Synonyms/Alias
 Adenylate cyclase-inhibiting G alpha protein 
Gene Name
 Gnai2 
Gene Synonyms/Alias
 Gnai-2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
29NLREDGEKAAREVKLubiquitination[1]
35EKAAREVKLLLLGAGubiquitination[1]
46LGAGESGKSTIVKQMubiquitination[1]
193VETHFTFKDLHFKMFubiquitination[1]
198TFKDLHFKMFDVGGQubiquitination[1]
249NRMHESMKLFDSICNubiquitination[1]
272IILFLNKKDLFEEKIubiquitination[1]
296PEYTGANKYDEAASYubiquitination[1]
307AASYIQSKFEDLNKRubiquitination[1]
313SKFEDLNKRKDTKEIubiquitination[1]
350VIIKNNLKDCGLF**ubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division. 
Sequence Annotation
 NP_BIND 40 47 GTP (By similarity).
 NP_BIND 176 182 GTP (By similarity).
 NP_BIND 201 205 GTP (By similarity).
 NP_BIND 270 273 GTP (By similarity).
 METAL 47 47 Magnesium (By similarity).
 METAL 182 182 Magnesium (By similarity).
 BINDING 327 327 GTP; via amide nitrogen (By similarity).
 LIPID 2 2 N-myristoyl glycine (By similarity).
 LIPID 3 3 S-palmitoyl cysteine (By similarity).  
Keyword
 Cell cycle; Cell division; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; GTP-binding; Lipoprotein; Magnesium; Membrane; Metal-binding; Myristate; Nucleotide-binding; Palmitate; Reference proteome; Transducer. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 355 AA 
Protein Sequence
MGCTVSAEDK AAAERSKMID KNLREDGEKA AREVKLLLLG AGESGKSTIV KQMKIIHEDG 60
YSEEECRQYR AVVYSNTIQS IMAIVKAMGN LQIDFADPQR ADDARQLFAL SCAAEEQGML 120
PEDLSGVIRR LWADHGVQAC FGRSREYQLN DSAAYYLNDL ERIAQSDYIP TQQDVLRTRV 180
KTTGIVETHF TFKDLHFKMF DVGGQRSERK KWIHCFEGVT AIIFCVALSA YDLVLAEDEE 240
MNRMHESMKL FDSICNNKWF TDTSIILFLN KKDLFEEKIT QSSLTICFPE YTGANKYDEA 300
ASYIQSKFED LNKRKDTKEI YTHFTCATDT KNVQFVFDAV TDVIIKNNLK DCGLF 355 
Gene Ontology
 GO:0005813; C:centrosome; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005834; C:heterotrimeric G-protein complex; IBA:RefGenome.
 GO:0045121; C:membrane raft; IEA:Compara.
 GO:0030496; C:midbody; ISS:UniProtKB.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:RefGenome.
 GO:0001664; F:G-protein coupled receptor binding; IBA:RefGenome.
 GO:0005525; F:GTP binding; ISA:MGI.
 GO:0003924; F:GTPase activity; IBA:RefGenome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004871; F:signal transducer activity; IBA:RefGenome.
 GO:0000186; P:activation of MAPKK activity; IEA:Compara.
 GO:0001973; P:adenosine receptor signaling pathway; IBA:RefGenome.
 GO:0007193; P:adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway; IMP:MGI.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; ISS:UniProtKB.
 GO:0008283; P:cell proliferation; IPI:MGI.
 GO:0007213; P:G-protein coupled acetylcholine receptor signaling pathway; IMP:MGI.
 GO:0007214; P:gamma-aminobutyric acid signaling pathway; IBA:RefGenome.
 GO:0007243; P:intracellular protein kinase cascade; IEA:Compara.
 GO:0050805; P:negative regulation of synaptic transmission; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0051924; P:regulation of calcium ion transport; IEA:Compara. 
Interpro
 IPR001408; Gprotein_alpha_I.
 IPR001019; Gprotein_alpha_su.
 IPR011025; GproteinA_insert.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00503; G-alpha 
SMART
 SM00275; G_alpha 
PROSITE
  
PRINTS
 PR00318; GPROTEINA.
 PR00441; GPROTEINAI.