CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005067
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Multifunctional protein ADE2 
Protein Synonyms/Alias
 Phosphoribosylaminoimidazole-succinocarboxamide synthase; SAICAR synthetase; Phosphoribosylaminoimidazole carboxylase; AIR carboxylase; AIRC 
Gene Name
 PAICS 
Gene Synonyms/Alias
 ADE2; AIRC; PAIS 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11AEVLNIGKKLYEGKTubiquitination[1, 2, 3]
12EVLNIGKKLYEGKTKubiquitination[3]
19KLYEGKTKEVYELLDubiquitination[3, 4]
30ELLDSPGKVLLQSKDubiquitination[3, 4, 5, 6]
36GKVLLQSKDQITAGNubiquitination[2, 3, 4, 5, 6, 7]
53RKNHLEGKAAISNKIacetylation[8]
53RKNHLEGKAAISNKIubiquitination[3, 4, 7]
74LLQEAGIKTAFTRKCubiquitination[3, 4, 7]
80IKTAFTRKCGETAFIubiquitination[3]
110IATGSFLKRNPGVKEubiquitination[2, 3, 4, 5, 6, 7]
116LKRNPGVKEGYKFYPubiquitination[3, 4]
125GYKFYPPKVELFFKDubiquitination[5, 6]
131PKVELFFKDDANNDPubiquitination[4]
235KQSYRDLKEVTPEGLubiquitination[3]
246PEGLQMVKKNFEWVAubiquitination[2, 3, 4]
247EGLQMVKKNFEWVAEacetylation[8]
247EGLQMVKKNFEWVAEubiquitination[3, 4]
261ERVELLLKSESQCRVubiquitination[3, 4]
283SDLGHCEKIKKACGNacetylation[8]
283SDLGHCEKIKKACGNubiquitination[3]
285LGHCEKIKKACGNFGubiquitination[3]
286GHCEKIKKACGNFGIubiquitination[2, 3]
304LRVTSAHKGPDETLRubiquitination[3, 4, 7]
313PDETLRIKAEYEGDGubiquitination[3, 4]
414LNTWISLKQADKKIRubiquitination[3, 4]
Reference
 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
  
Sequence Annotation
 REGION 2 260 SAICAR synthetase.
 REGION 261 266 Linker.
 REGION 267 425 AIR carboxylase.
 ACT_SITE 101 101 For SAICAR synthetase activity.
 ACT_SITE 107 107 For SAICAR synthetase activity.
 ACT_SITE 215 215 For SAICAR synthetase activity.
 ACT_SITE 303 303 For AIR carboxylase activity.
 ACT_SITE 332 332 For AIR carboxylase activity.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 22 22 Phosphotyrosine (By similarity).
 MOD_RES 27 27 Phosphoserine.
 MOD_RES 107 107 Phosphoserine.
 MOD_RES 238 238 Phosphothreonine.
 MOD_RES 247 247 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Decarboxylase; Direct protein sequencing; Ligase; Lyase; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Polymorphism; Purine biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 425 AA 
Protein Sequence
MATAEVLNIG KKLYEGKTKE VYELLDSPGK VLLQSKDQIT AGNAARKNHL EGKAAISNKI 60
TSCIFQLLQE AGIKTAFTRK CGETAFIAPQ CEMIPIEWVC RRIATGSFLK RNPGVKEGYK 120
FYPPKVELFF KDDANNDPQW SEEQLIAAKF CFAGLLIGQT EVDIMSHATQ AIFEILEKSW 180
LPQNCTLVDM KIEFGVDVTT KEIVLADVID NDSWRLWPSG DRSQQKDKQS YRDLKEVTPE 240
GLQMVKKNFE WVAERVELLL KSESQCRVVV LMGSTSDLGH CEKIKKACGN FGIPCELRVT 300
SAHKGPDETL RIKAEYEGDG IPTVFVAVAG RSNGLGPVMS GNTAYPVISC PPLTPDWGVQ 360
DVWSSLRLPS GLGCSTVLSP EGSAQFAAQI FGLSNHLVWS KLRASILNTW ISLKQADKKI 420
RECNL 425 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0034023; F:5-(carboxyamino)imidazole ribonucleotide mutase activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004638; F:phosphoribosylaminoimidazole carboxylase activity; TAS:Reactome.
 GO:0004639; F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; TAS:Reactome.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0009113; P:purine nucleobase biosynthetic process; TAS:ProtInc.
 GO:0009168; P:purine ribonucleoside monophosphate biosynthetic process; TAS:Reactome. 
Interpro
 IPR013816; ATP_grasp_subdomain_2.
 IPR000031; N5-CAIR_Mutase_PurE_dom.
 IPR001636; SAICAR_synth.
 IPR018236; SAICAR_synthetase_CS. 
Pfam
 PF00731; AIRC
 PF01259; SAICAR_synt 
SMART
 SM01001; AIRC 
PROSITE
 PS01057; SAICAR_SYNTHETASE_1
 PS01058; SAICAR_SYNTHETASE_2 
PRINTS